Projects per year
Abstract
Cohesins establish sister chromatid cohesion during S phase, and are removed when cohesin Scc1 is cleaved by separase at anaphase onset. During this process, cohesin Smc3 undergoes a cycle of acetylation: Smc3 acetylation by Eco1 in S phase stabilizes cohesin association with chromosomes, and its deacetylation by Hos1 in anaphase allows re-use of Smc3 in the next cell cycle. Here we find Smc3 deacetylation by Hos1 has a more immediate effect in early anaphase of budding yeast. Hos1 depletion significantly delayed sister chromatid separation and segregation. Smc3 deacetylation facilitated removal of cohesins from chromosomes, without changing Scc1 cleavage efficiency, promoting dissolution of cohesion. This action is probably due to disengagement of Smc1–3 heads, prompted by de-repression of their ATPase activity. We suggest Scc1 cleavage per se is insufficient for efficient dissolution of cohesion in early anaphase, but subsequent Smc3 deacetylation, triggered by Scc1 cleavage, is also required.
Original language | English |
---|---|
Pages (from-to) | 605-614 |
Number of pages | 10 |
Journal | Molecular Cell |
Volume | 68 |
Issue number | 3 |
DOIs | |
Publication status | Published - 2 Nov 2017 |
Fingerprint
Dive into the research topics of 'Smc3 Deacetylation by Hos1 Facilitates Efficient Dissolution of Sister Chromatid Cohesion during Early Anaphase'. Together they form a unique fingerprint.Projects
- 3 Finished
-
SELFCC: Chromosome Self-clearing Completes Sister Chromatid Separation
Tanaka, T. (Investigator)
COMMISSION OF THE EUROPEAN COMMUNITIES
1/04/13 → 30/09/18
Project: Research
-
Strategic Award: Wellcome Trust Technology Platform
Blow, J. (Investigator), Lamond, A. (Investigator) & Owen-Hughes, T. (Investigator)
1/01/13 → 30/09/18
Project: Research
-
Molecular Mechanisms Regulating the Kinetochore-Microtubule Interaction in Mitosis (Principal Research Fellowship)
Tanaka, T. (Investigator)
1/04/12 → 30/04/21
Project: Research
Datasets
-
Smc3 deacetylation by Hos1 facilitates efficient dissolution of sister chromatid cohesion during early anaphase
Yue, Z. (Contributor), Tanaka, T. (Creator) & Li, S. (Contributor), Mendeley Data, 4 Nov 2017
DOI: 10.17632/m64mz2tbvh.2, https://data.mendeley.com/datasets/m64mz2tbvh
Dataset
Profiles
-
Tanaka, Tomoyuki
- Molecular Cell and Developmental Biology - Professor of Cell and Molecular Biology
Person: Academic