SNIP1 is a candidate modifier of the transcriptional activity of c-Myc on E box-dependent target genes

Makiko Fujii, Lyudmila A. Lyakh, Cameron P. Bracken, Junya Fukuoka, Morisada Hayakawa, Tadasuke Tsukiyama, Steven J. Soll, Melissa Harris, Sonia Rocha, Kevin C. Roche, Shin-ichi Tominaga, Jin Jen, Neil D. Perkins, Robert J. Lechleider, Anita B. Roberts

    Research output: Contribution to journalArticlepeer-review

    41 Citations (Scopus)

    Abstract

    Using a yeast two-hybrid screen, we found that SNIP1 (Smad nuclear-interacting protein 1) associates with c-Myc, a key regulator of cell proliferation and transformation. We demonstrate that SNIP1 functions as an important regulator of c-Myc activity, binding the N terminus of c-Myc through its own C terminus, and that SNIP1 enhances the transcriptional activity of c-Myc both by stabilizing it against proteosomal degradation and by bridging the c-Myc/p300 complex. These effects of SNIP1 on c-Myc likely contribute to synergistic effects of SNIP1, c-Myc, and H-Ras in inducing formation of foci in an in vitro transformation assay and also in supporting anchorage-independent growth. The significant association of SNIP1 and c-Myc staining in a non-small cell lung cancer tissue array is further evidence that their activities might be linked and suggests that SNIP1 might be an important modulator of c-Myc activity in carcinogenesis.
    Original languageEnglish
    Pages (from-to)771-83
    Number of pages13
    JournalMolecular Cell
    Volume24
    Issue number5
    DOIs
    Publication statusPublished - 8 Dec 2006

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