SNIP1 is a candidate modifier of the transcriptional activity of c-Myc on E box-dependent target genes

TY - JOUR

T1 - SNIP1 is a candidate modifier of the transcriptional activity of c-Myc on E box-dependent target genes

AU - Fujii, Makiko

AU - Lyakh, Lyudmila A.

AU - Bracken, Cameron P.

AU - Fukuoka, Junya

AU - Hayakawa, Morisada

AU - Tsukiyama, Tadasuke

AU - Soll, Steven J.

AU - Harris, Melissa

AU - Rocha, Sonia

AU - Roche, Kevin C.

AU - Tominaga, Shin-ichi

AU - Jen, Jin

AU - Perkins, Neil D.

AU - Lechleider, Robert J.

AU - Roberts, Anita B.

PY - 2006/12/8

Y1 - 2006/12/8

N2 - Using a yeast two-hybrid screen, we found that SNIP1 (Smad nuclear-interacting protein 1) associates with c-Myc, a key regulator of cell proliferation and transformation. We demonstrate that SNIP1 functions as an important regulator of c-Myc activity, binding the N terminus of c-Myc through its own C terminus, and that SNIP1 enhances the transcriptional activity of c-Myc both by stabilizing it against proteosomal degradation and by bridging the c-Myc/p300 complex. These effects of SNIP1 on c-Myc likely contribute to synergistic effects of SNIP1, c-Myc, and H-Ras in inducing formation of foci in an in vitro transformation assay and also in supporting anchorage-independent growth. The significant association of SNIP1 and c-Myc staining in a non-small cell lung cancer tissue array is further evidence that their activities might be linked and suggests that SNIP1 might be an important modulator of c-Myc activity in carcinogenesis.

AB - Using a yeast two-hybrid screen, we found that SNIP1 (Smad nuclear-interacting protein 1) associates with c-Myc, a key regulator of cell proliferation and transformation. We demonstrate that SNIP1 functions as an important regulator of c-Myc activity, binding the N terminus of c-Myc through its own C terminus, and that SNIP1 enhances the transcriptional activity of c-Myc both by stabilizing it against proteosomal degradation and by bridging the c-Myc/p300 complex. These effects of SNIP1 on c-Myc likely contribute to synergistic effects of SNIP1, c-Myc, and H-Ras in inducing formation of foci in an in vitro transformation assay and also in supporting anchorage-independent growth. The significant association of SNIP1 and c-Myc staining in a non-small cell lung cancer tissue array is further evidence that their activities might be linked and suggests that SNIP1 might be an important modulator of c-Myc activity in carcinogenesis.

U2 - 10.1016/j.molcel.2006.11.006

DO - 10.1016/j.molcel.2006.11.006

M3 - Article

C2 - 17157259

SN - 1097-2765

VL - 24

SP - 771

EP - 783

JO - Molecular Cell

JF - Molecular Cell

IS - 5

ER -