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Abstract
The type six secretion system (T6SS) is a transmembrane protein complex that mediates bacterial cell killing. The T6SS comprises three main components (transmembrane, baseplate and sheath/tube complexes) that are sequentially assembled in order to enable an attacking cell to transport payloads into neighbouring cells. A T6SS attack disrupts the function of essential cellular components of target cells, typically resulting in their death. While the assembled T6SS adopts a fixed position in the cell membrane of the attacking cell, the location of the firing site varies between firing events. In Serratia marcescens, a post-translational regulatory network regulates the assembly and firing kinetics of the T6SS in a manner that affects the attacking cell's ability to kill target cells. Moreover, when the ability of membrane complexes to reorient is reduced, an attacking cell's competitiveness is also reduced. In this study, we will develop a mathematical model that describes both the spatial motion and assembly/disassembly of a firing T6SS. The model represents the motion of a T6SS on the cell membrane as a state-dependent random walk. Using the model, we will explore how both spatial and temporal effects can combine to give rise to different firing phenotypes. Using parameters inferred from the available literature, we show that variation in estimated diffusion coefficients is sufficient to give rise to either spatially local or global firers.
Original language | English |
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Article number | 230284 |
Number of pages | 10 |
Journal | Royal Society Open Science |
Volume | 10 |
Issue number | 11 |
DOIs | |
Publication status | Published - 1 Nov 2023 |
Keywords
- type six secretion system
- spatio-temporal modelling
- random walk
ASJC Scopus subject areas
- General
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Dive into the research topics of 'Space and time on the membrane: modelling Type VI secretion system dynamics as a state-dependent random walk'. Together they form a unique fingerprint.Projects
- 1 Finished
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Institutional Strategic Support Fund (2016)
Cantrell, D. (Investigator), Dinkova-Kostova, A. (Investigator), Kang, L. (Investigator), Murray, P. (Investigator) & Pearson, E. (Investigator)
1/10/16 → 31/05/23
Project: Research