Spatial monitoring of toxicity in HMOX-LacZ transgenic mice

Rachel Young, C. Roland Wolf, Ken Brown, John D. Hayes, C. Bruce A. Whitelaw

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    4 Citations (Scopus)

    Abstract

    Transgenic reporter mice can contribute in the development of less toxic and more selective drugs to treat disease. In this brief communication we describe the generation and initial validation of transgenic mice that provide a visual spatial readout of oxidative stress. These mice carry a LacZ reporter transgene driven by the human haem oxygenase 1 promoter. The induction of LacZ staining by a range of compounds indicated differences in the haem oxygenase 1 spatial response within a tissue. Thus this transgene allows for the spatial monitoring of differences in toxic insult and indicates that this type of transgenic system could have use in toxicity screens.

    Original languageEnglish
    Pages (from-to)897-902
    Number of pages6
    JournalTransgenic Research
    Volume19
    Issue number5
    DOIs
    Publication statusPublished - Oct 2010

    Keywords

    • Etoposide
    • Hypoxia
    • Mice
    • Toxin Transgene
    • HEME OXYGENASE-1 GENE
    • CARBON-MONOXIDE
    • TRANSCRIPTIONAL ACTIVATION
    • EXPRESSION
    • PROTEIN
    • LIPOPOLYSACCHARIDE
    • INDUCTION
    • BILIRUBIN
    • CELLS
    • MACROPHAGES

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