Spatial regulation and organization of DNA replication within the nucleus

Toyoaki Natsume, Tomoyuki U. Tanaka

    Research output: Contribution to journalArticle

    21 Citations (Scopus)

    Abstract

    Duplication of chromosomal DNA is a temporally and spatially regulated process. The timing of DNA replication initiation at various origins is highly coordinated; some origins fire early and others late during S phase. Moreover, inside the nuclei, the bulk of DNA replication is physically organized in replication factories, consisting of DNA polymerases and other replication proteins. In this review article, we discuss how DNA replication is organized and regulated spatially within the nucleus and how this spatial organization is linked to temporal regulation. We focus on DNA replication in budding yeast and fission yeast and, where applicable, compare yeast DNA replication with that in bacteria and metazoans.

    Original languageEnglish
    Pages (from-to)7-17
    Number of pages11
    JournalChromosome Research
    Volume18
    Issue number1
    DOIs
    Publication statusPublished - Jan 2010

    Keywords

    • DNA replication
    • replication origin
    • replication fork
    • replisome
    • replicon
    • replication focus
    • replication factory
    • XENOPUS EGG EXTRACTS
    • SACCHAROMYCES-CEREVISIAE
    • S-PHASE
    • CELL-CYCLE
    • CHROMOSOME-REPLICATION
    • YEAST TELOMERES
    • HIGH-RESOLUTION
    • BUDDING YEAST
    • FISSION YEAST
    • HISTONE DEACETYLASE

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