Spatially optimized gene transfection by laser-induced breakdown of optically trapped nanoparticles

Yoshihiko Arita, Maria Leilani Torres-Mapa, Woei Ming Lee, Tomas Cizmar, Paul Campbell, Frank J. Gunn-Moore, Kishan Dholakia

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    33 Citations (Scopus)

    Abstract

    We demonstrate laser-induced breakdown of an optically trapped nanoparticle with a nanosecond laser pulse. Controllable cavitation within a microscope sample was achieved, generating shear stress to monolayers of live cells. This efficiently permeabilize their plasma membranes. We show that this technique is an excellent tool for plasmid-DNA transfection of cells with both reduced energy requirements and reduced cell lysis compared to previously reported approaches. Simultaneous multisite targeted nanosurgery of cells is also demonstrated using a spatial light modulator for parallelizing the technique.

    Original languageEnglish
    Article number093702
    Number of pages3
    JournalApplied Physics Letters
    Volume98
    Issue number9
    DOIs
    Publication statusPublished - 2 Mar 2011

    Keywords

    • INDUCED CELL-LYSIS
    • CAVITATION BUBBLES
    • SONOPORATION
    • GENERATION
    • COLLAPSE
    • WAVES

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