Spatially optimized gene transfection by laser-induced breakdown of optically trapped nanoparticles

TY - JOUR

T1 - Spatially optimized gene transfection by laser-induced breakdown of optically trapped nanoparticles

AU - Arita, Yoshihiko

AU - Torres-Mapa, Maria Leilani

AU - Lee, Woei Ming

AU - Cizmar, Tomas

AU - Campbell, Paul

AU - Gunn-Moore, Frank J.

AU - Dholakia, Kishan

PY - 2011/3/2

Y1 - 2011/3/2

N2 - We demonstrate laser-induced breakdown of an optically trapped nanoparticle with a nanosecond laser pulse. Controllable cavitation within a microscope sample was achieved, generating shear stress to monolayers of live cells. This efficiently permeabilize their plasma membranes. We show that this technique is an excellent tool for plasmid-DNA transfection of cells with both reduced energy requirements and reduced cell lysis compared to previously reported approaches. Simultaneous multisite targeted nanosurgery of cells is also demonstrated using a spatial light modulator for parallelizing the technique.

AB - We demonstrate laser-induced breakdown of an optically trapped nanoparticle with a nanosecond laser pulse. Controllable cavitation within a microscope sample was achieved, generating shear stress to monolayers of live cells. This efficiently permeabilize their plasma membranes. We show that this technique is an excellent tool for plasmid-DNA transfection of cells with both reduced energy requirements and reduced cell lysis compared to previously reported approaches. Simultaneous multisite targeted nanosurgery of cells is also demonstrated using a spatial light modulator for parallelizing the technique.

KW - INDUCED CELL-LYSIS

KW - CAVITATION BUBBLES

KW - SONOPORATION

KW - GENERATION

KW - COLLAPSE

KW - WAVES

U2 - 10.1063/1.3554415

DO - 10.1063/1.3554415

M3 - Article

SN - 0003-6951

VL - 98

JO - Applied Physics Letters

JF - Applied Physics Letters

IS - 9

M1 - 093702

ER -