Projects per year
Axolotls are uniquely able to resolve spinal cord injuries, but little is known about the mechanisms underlying spinal cord regeneration. We previously found that tail amputation leads to reactivation of a developmental-like program in spinal cord ependymal cells (Rodrigo Albors et al., 2015), characterized by a high-proliferation zone emerging 4 days post-amputation (Rost et al., 2016). What underlies this spatiotemporal pattern of cell proliferation, however, remained unknown. Here, we use modeling, tightly linked to experimental data, to demonstrate that this regenerative response is consistent with a signal that recruits ependymal cells during ~85 hours after amputation within ~830 μm of the injury. We adapted Fluorescent Ubiquitination-based Cell Cycle Indicator (FUCCI) technology to axolotls (AxFUCCI) to visualize cell cycles in vivo. AxFUCCI axolotls confirmed the predicted appearance time and size of the injury-induced recruitment zone and revealed cell cycle synchrony between ependymal cells. Our modeling and imaging move us closer to understanding bona fide spinal cord regeneration.
- computational biology
- developmental biology
- systems biology
- cell proliferation
- cell cycle
- Computational and Systems Biology
- computational model
- Research Advance
- spinal cord regeneration
- Developmental Biology
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Immunology and Microbiology(all)
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- 1 Finished
Dissecting Spinal Cord Ependymal Cell Heterongeneity by Single-Cell Transciptomics (ECtomics) (Joint with MRC Institute of Genetics and Molecular Medicine and Sanger-EBI Single Cell Genomics Centre)
Rodrigo Albors, A. & Storey, K.
1/04/17 → 31/03/19