Specific regulation of mRNA cap methylation by the c-Myc and E2F1 transcription factors

M. D. Cole, V. H. Cowling

    Research output: Contribution to journalArticle

    46 Citations (Scopus)

    Abstract

    Methylation of the mRNA 50 guanosine cap is essential for efficient gene expression. The 50 methyl cap binds to eIF4E, which is the first step in the recruitment of mRNA to the 40S ribosomal subunit. To investigate whether mRNA cap methylation is regulated in a gene-specific manner, we established a method to detect the relative level of cap methylation on specific mRNAs. We found that two transcription factors, c-Myc and E2F1, induce cap methylation of their transcriptional target genes, and therefore, c-Myc and E2F1 upregulate gene expression by simultaneously inducing transcription and promoting translation. c-Myc-induced cap methylation is greater than transcriptional induction for the majority of its target genes, indicating that this is a major mechanism by which Myc regulates gene expression.

    Original languageEnglish
    Pages (from-to)1169-1175
    Number of pages7
    JournalOncogene
    Volume28
    Issue number9
    DOIs
    Publication statusPublished - 5 Mar 2009

    Keywords

    • E2F1
    • gene expression
    • mRNA cap methylation
    • Myc
    • translation
    • CONDITIONAL MUTANTS
    • POLYMERASE-II
    • GENES
    • EXPRESSION
    • TRANSLATION
    • BINDING
    • IDENTIFICATION
    • ONCOPROTEINS
    • RECRUITMENT
    • ACTIVATION

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