Sperm Cell Painting: A Mechanism Driven Approach for Drug Discovery in Human Spermatozoa

Zoe Johnston; Franz Gruber; Rachel Myles; Shruti Kane; Anthony Richardson; David Day; Caroline Wilson; Kevin Read; Ian Gilbert; Sarah Martins Da Silva; Christopher Barratt; Jason Swedlow

doi:10.1101/2023.09.15.557919

Sperm Cell Painting: A Mechanism Driven Approach for Drug Discovery in Human Spermatozoa

Zoe Johnston, Franz Gruber, Rachel Myles, Shruti Kane, Anthony Richardson, David Day, Caroline Wilson, Kevin Read, Ian Gilbert, Sarah Martins Da Silva, Christopher Barratt, Jason Swedlow (Lead / Corresponding author)

Research output: Working paper/Preprint › Preprint

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Abstract

We have adapted the cell painting assay developed by Carpenter and colleagues on cultured U2OS cells to human spermatozoa. In Sperm Cell Painting (SCP) we assemble an image-based quantitative fingerprint of the functional state of sperm. We use this assay to gain insight into the mechanism of action of compounds that modify sperm function and as a platform for contraceptive discovery.

Original language	English
Publisher	BioRxiv
Number of pages	20
DOIs	https://doi.org/10.1101/2023.09.15.557919
Publication status	Published - 17 Sept 2023

Keywords

Drug discovery
Sperm biology
high throughput screening
spermatozoa
Cell Painting
whole cell visualisation

Access to Document

10.1101/2023.09.15.557919Licence: CC BY

Pre-printSubmitted manuscript, 1.76 MBLicence: CC BY

Drug discovery and sperm: advancing the frontiers.
Johnston, Z. (Invited speaker)
31 Aug 2024
Activity: Talk or presentation types › Invited talk

SRF Poster Prize Winner 2024 - runner up
Johnston, Z. (Recipient), 13 Jan 2024
Prize: Prize (including medals and awards)

Cite this

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title = "Sperm Cell Painting: A Mechanism Driven Approach for Drug Discovery in Human Spermatozoa",

abstract = "We have adapted the cell painting assay developed by Carpenter and colleagues on cultured U2OS cells to human spermatozoa. In Sperm Cell Painting (SCP) we assemble an image-based quantitative fingerprint of the functional state of sperm. We use this assay to gain insight into the mechanism of action of compounds that modify sperm function and as a platform for contraceptive discovery.",

keywords = "Drug discovery, Sperm biology, high throughput screening, spermatozoa, Cell Painting, whole cell visualisation",

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AU - Johnston, Zoe

AU - Gruber, Franz

AU - Myles, Rachel

AU - Kane, Shruti

AU - Richardson, Anthony

AU - Day, David

AU - Wilson, Caroline

AU - Read, Kevin

AU - Gilbert, Ian

AU - Martins Da Silva, Sarah

AU - Barratt, Christopher

AU - Swedlow, Jason

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N2 - We have adapted the cell painting assay developed by Carpenter and colleagues on cultured U2OS cells to human spermatozoa. In Sperm Cell Painting (SCP) we assemble an image-based quantitative fingerprint of the functional state of sperm. We use this assay to gain insight into the mechanism of action of compounds that modify sperm function and as a platform for contraceptive discovery.

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KW - Drug discovery

KW - Sperm biology

KW - high throughput screening

KW - spermatozoa

KW - Cell Painting

KW - whole cell visualisation

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DO - 10.1101/2023.09.15.557919

M3 - Preprint

BT - Sperm Cell Painting

PB - BioRxiv

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Sperm Cell Painting: A Mechanism Driven Approach for Drug Discovery in Human Spermatozoa

Abstract

Keywords

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Activities

Drug discovery and sperm: advancing the frontiers.

Prizes

SRF Poster Prize Winner 2024 - runner up

Cite this