Sperm Cell Painting: A Mechanism Driven Approach for Drug Discovery in Human Spermatozoa

TY - UNPB

T1 - Sperm Cell Painting

T2 - A Mechanism Driven Approach for Drug Discovery in Human Spermatozoa

AU - Johnston, Zoe

AU - Gruber, Franz

AU - Myles, Rachel

AU - Kane, Shruti

AU - Richardson, Anthony

AU - Day, David

AU - Wilson, Caroline

AU - Read, Kevin

AU - Gilbert, Ian

AU - Martins Da Silva, Sarah

AU - Barratt, Christopher

AU - Swedlow, Jason

N1 - The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.

PY - 2023/9/17

Y1 - 2023/9/17

N2 - We have adapted the cell painting assay developed by Carpenter and colleagues on cultured U2OS cells to human spermatozoa. In Sperm Cell Painting (SCP) we assemble an image-based quantitative fingerprint of the functional state of sperm. We use this assay to gain insight into the mechanism of action of compounds that modify sperm function and as a platform for contraceptive discovery.

AB - We have adapted the cell painting assay developed by Carpenter and colleagues on cultured U2OS cells to human spermatozoa. In Sperm Cell Painting (SCP) we assemble an image-based quantitative fingerprint of the functional state of sperm. We use this assay to gain insight into the mechanism of action of compounds that modify sperm function and as a platform for contraceptive discovery.

KW - Drug discovery

KW - Sperm biology

KW - high throughput screening

KW - spermatozoa

KW - Cell Painting

KW - whole cell visualisation

U2 - 10.1101/2023.09.15.557919

DO - 10.1101/2023.09.15.557919

M3 - Preprint

BT - Sperm Cell Painting

PB - BioRxiv

ER -