Projects per year
Abstract
Male contraceptive options and infertility treatments are limited, and almost all innovation has been limited to updates to medically assisted reproduction protocols and methods. To accelerate the development of drugs that can either improve or inhibit fertility, we established a small molecule library as a toolbox for assay development and screening campaigns using human spermatozoa. We have profiled all compounds in the Sperm Toolbox in several automated high-throughput assays that measure stimulation or inhibition of sperm motility or the acrosome reaction. We have assayed motility under non-capacitating and capacitating conditions to distinguish between pathways operating under these different physiological states. We also assayed cell viability to ensure any effects on sperm function are specific. A key advantage of our studies is that all compounds are assayed together in the same experimental conditions, which allows quantitative comparisons of their effects in complementary functional assays. We have combined the resulting datasets to generate fingerprints of the Sperm Toolbox compounds on sperm function. The data are included in an on-line R-based app for convenient querying.
Original language | English |
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Article number | e0297666 |
Number of pages | 16 |
Journal | PLoS ONE |
Volume | 19 |
Issue number | 2 |
DOIs | |
Publication status | Published - 20 Feb 2024 |
Keywords
- Humans
- Male
- Sperm Motility
- Semen
- Spermatozoa/metabolism
- Acrosome Reaction
- Fertility
ASJC Scopus subject areas
- General
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Dive into the research topics of 'Sperm Toolbox-A selection of small molecules to study human spermatozoa'. Together they form a unique fingerprint.Projects
- 1 Finished
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Male Contraceptive Drug Development Using a Novel Human Sperm Phenotypic Screening Platform (Grand Challenges Phase II)
Andrews, P. (Investigator), Barratt, C. (Investigator), Gilbert, I. (Investigator), Martins Da Silva, S. (Investigator), Read, K. (Investigator) & Swedlow, J. (Investigator)
Bill and Melinda Gates Foundation
1/11/18 → 30/09/23
Project: Research