Splicing factors facilitate RNAi-directed silencing in fission yeast

Elizabeth H. Bayne, Manuela Portoso, Alexander Kagansky, Isabelle C. Kos-Braun, Takeshi Urano, Karl Ekwall, Flavia Alves, Juri Rappsilber, Robin C. Allshire

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    95 Citations (Scopus)

    Abstract

    Heterochromatin formation at fission yeast centromeres is directed by RNA interference (RNAi). Noncoding transcripts derived from centromeric repeats are processed into small interfering RNAs (siRNAs) that direct the RNA-induced transcriptional silencing (RITS) effector complex to engage centromer transcripts, resulting in recruitment of the histone H3 lysine 9 methyltransferase Clr4, and hence silencing. We have found that defects in specific splicing factors, but not splicing itself, affect the generation of centromeric siRNAs and consequently centromeric heterochromatin integrity. Moreover, splicing factors physically associate with Cid12, a component of the RNAi machinery, and with centromeric chromatin, consistent with a direct role in RNAi. We propose that spliceosomal complexes provide a platform for siRNA generation and hence facilitate effective centromere repeat silencing.

    Original languageEnglish
    Pages (from-to)602-606
    Number of pages5
    JournalScience
    Volume322
    Issue number5901
    DOIs
    Publication statusPublished - 24 Oct 2008

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