Spontaneous release of immunoreactive neuropeptide y from the central terminals of large diameter primary afferents of rats with peripheral nerve injury

M. A. Mark, L. A. Colvin, A. W. Duggan (Lead / Corresponding author)

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23 Citations (Scopus)


Microprobes bearing immobilized antibodies to the C-terminus of neuropeptide Y were used to measure the release of this neuropeptide in the spinal cords of rats with a unilateral peripheral neuropathy and in sham- operated animals. All neuropathic animals showed the characteristic behavioural syndrome and were studied at 14 days postsciatic nerve loose- ligation. An extensive spontaneous release of immunoreactive neuropeptide Y was detected in the spinal cords of the neuropathic rats and, compared to sham-operated rats, a new zone of release was found in the deep dorsal horn. Electrical stimulation of large diameter primary afferents proximal to the nerve ligature produced widespread release of neuropeptide Y in the dorsal horn which persisted for up to 1 h poststimulation. It is possible that ectopic impulses arising in the injured nerve were responsible for the spontaneous central release of neuropeptide Y and this neuropeptide may play a role in the central response to peripheral nerve injury.

Original languageEnglish
Pages (from-to)581-589
Number of pages9
Issue number2
Early online date24 Jun 1998
Publication statusPublished - 1998



  • Antibody microprobes
  • Neuropathic rat
  • Neuropeptide Y release
  • Spinal cord

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