Spontaneous release of immunoreactive neuropeptide y from the central terminals of large diameter primary afferents of rats with peripheral nerve injury

TY - JOUR

T1 - Spontaneous release of immunoreactive neuropeptide y from the central terminals of large diameter primary afferents of rats with peripheral nerve injury

AU - Mark, M. A.

AU - Colvin, L. A.

AU - Duggan, A. W.

PY - 1998

Y1 - 1998

N2 - Microprobes bearing immobilized antibodies to the C-terminus of neuropeptide Y were used to measure the release of this neuropeptide in the spinal cords of rats with a unilateral peripheral neuropathy and in sham- operated animals. All neuropathic animals showed the characteristic behavioural syndrome and were studied at 14 days postsciatic nerve loose- ligation. An extensive spontaneous release of immunoreactive neuropeptide Y was detected in the spinal cords of the neuropathic rats and, compared to sham-operated rats, a new zone of release was found in the deep dorsal horn. Electrical stimulation of large diameter primary afferents proximal to the nerve ligature produced widespread release of neuropeptide Y in the dorsal horn which persisted for up to 1 h poststimulation. It is possible that ectopic impulses arising in the injured nerve were responsible for the spontaneous central release of neuropeptide Y and this neuropeptide may play a role in the central response to peripheral nerve injury.

AB - Microprobes bearing immobilized antibodies to the C-terminus of neuropeptide Y were used to measure the release of this neuropeptide in the spinal cords of rats with a unilateral peripheral neuropathy and in sham- operated animals. All neuropathic animals showed the characteristic behavioural syndrome and were studied at 14 days postsciatic nerve loose- ligation. An extensive spontaneous release of immunoreactive neuropeptide Y was detected in the spinal cords of the neuropathic rats and, compared to sham-operated rats, a new zone of release was found in the deep dorsal horn. Electrical stimulation of large diameter primary afferents proximal to the nerve ligature produced widespread release of neuropeptide Y in the dorsal horn which persisted for up to 1 h poststimulation. It is possible that ectopic impulses arising in the injured nerve were responsible for the spontaneous central release of neuropeptide Y and this neuropeptide may play a role in the central response to peripheral nerve injury.

KW - Antibody microprobes

KW - Neuropathic rat

KW - Neuropeptide Y release

KW - Spinal cord

UR - https://www.scopus.com/pages/publications/0031929926

U2 - 10.1016/S0306-4522(97)00402-8

DO - 10.1016/S0306-4522(97)00402-8

M3 - Article

C2 - 9460764

AN - SCOPUS:0031929926

SN - 0306-4522

VL - 83

SP - 581

EP - 589

JO - Neuroscience

JF - Neuroscience

IS - 2

ER -