Spray-Induced Gene Silencing as a Potential Tool to Control Potato Late Blight Disease

Pruthvi B. Kalyandurg, Poorva Sundararajan, Mukesh Dubey, Farideh Ghadamgahi, Muhammad Awais Zahid, Stephen C. Whisson, Ramesh R. Vetukuri (Lead / Corresponding author)

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)
75 Downloads (Pure)

Abstract

Phytophthora infestans causes late blight disease on potato and tomato and is currently controlled by resistant cultivars or intensive fungicide spraying. Here, we investigated an alternative means for late blight control by spraying potato leaves with double-stranded RNAs (dsRNA) that target the P. infestans genes essential for infection. First, we showed that the sporangia of P. infestans expressing green fluorescent protein (GFP) can take up in vitro synthesized dsRNAs homologous to GFP directly from their surroundings, including leaves, which led to the reduced relative expression of GFP. We further demonstrate the potential of spray-induced gene silencing (SIGS) in controlling potato late blight disease by targeting developmentally important genes in P. infestans such as guanine-nucleotide binding protein β-subunit (PiGPB1), haustorial membrane protein (PiHmp1), cutinase (PiCut3), and endo-1,3(4)-β-glucanase (PiEndo3). Our results demonstrate that SIGS can potentially be used to mitigate potato late blight; however, the degree of disease control is dependent on the selection of the target genes.[Formula: see text] Copyright © 2021 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.

Original languageEnglish
Pages (from-to)2168-2175
Number of pages8
JournalPhytopathology
Volume111
Issue number12
Early online date2 Dec 2021
DOIs
Publication statusPublished - Dec 2021

Keywords

  • Gene Silencing
  • Phytophthora infestans
  • Plant Diseases
  • Solanum tuberosum/genetics
  • Sporangia

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