Spray-Induced Gene Silencing to Study Gene Function in Phytophthora

Poorva Sundararajan; Pruthvi B. Kalyandurg; Qinsong Liu; Aakash Chawade; Stephen C. Whisson; Ramesh R. Vetukuri

doi:10.1007/978-1-0716-2517-0_27

Spray-Induced Gene Silencing to Study Gene Function in Phytophthora

Poorva Sundararajan, Pruthvi B. Kalyandurg, Qinsong Liu, Aakash Chawade, Stephen C. Whisson, Ramesh R. Vetukuri (Lead / Corresponding author)

DArcy Thompson Unit

Research output: Chapter in Book/Report/Conference proceeding › Chapter (peer-reviewed) › peer-review

1 Citation (Scopus)

Abstract

RNA interference (RNAi) is a conserved cellular defense mechanism mediated by double-stranded RNA (dsRNA) that can regulate gene expression through targeted destruction of mRNAs (messenger RNAs). Recent studies have shown that spraying dsRNAs or small RNAs (sRNAs) that target essential genes of pathogens on plant surfaces can confer protection against pests and pathogens. Also called spray-induced gene silencing (SIGS), this strategy can be used for disease control and for transient gene silencing to study the function of genes in plant-pathogen interactions. Furthermore, as sRNAs can move locally, systemically, and cross-kingdom during plant-microbe interactions, SIGS allows quick detection and characterization of gene functions in pathogens and plants.

Original language	English
Title of host publication	Plant Pathology
Subtitle of host publication	Method and Protocols
Editors	Nicola Luchi
Place of Publication	New York
Publisher	Humana Press
Pages	459-474
Number of pages	16
Edition	1
ISBN (Electronic)	9781071625170
ISBN (Print)	9781071625163
DOIs	https://doi.org/10.1007/978-1-0716-2517-0_27
Publication status	Published - 2022

Publication series

Name	Methods in Molecular Biology
Volume	2536
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Keywords

Gene silencing
Oomycetes
Potato
RNAi
SIGS

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-0716-2517-0_27

Cite this

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abstract = "RNA interference (RNAi) is a conserved cellular defense mechanism mediated by double-stranded RNA (dsRNA) that can regulate gene expression through targeted destruction of mRNAs (messenger RNAs). Recent studies have shown that spraying dsRNAs or small RNAs (sRNAs) that target essential genes of pathogens on plant surfaces can confer protection against pests and pathogens. Also called spray-induced gene silencing (SIGS), this strategy can be used for disease control and for transient gene silencing to study the function of genes in plant-pathogen interactions. Furthermore, as sRNAs can move locally, systemically, and cross-kingdom during plant-microbe interactions, SIGS allows quick detection and characterization of gene functions in pathogens and plants.",

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Spray-Induced Gene Silencing to Study Gene Function in Phytophthora. / Sundararajan, Poorva; Kalyandurg, Pruthvi B.; Liu, Qinsong et al.
Plant Pathology: Method and Protocols. ed. / Nicola Luchi. 1. ed. New York: Humana Press, 2022. p. 459-474 (Methods in Molecular Biology; Vol. 2536).

Research output: Chapter in Book/Report/Conference proceeding › Chapter (peer-reviewed) › peer-review

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Spray-Induced Gene Silencing to Study Gene Function in Phytophthora

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