Spray-Induced Gene Silencing to Study Gene Function in Phytophthora

Poorva Sundararajan, Pruthvi B. Kalyandurg, Qinsong Liu, Aakash Chawade, Stephen C. Whisson, Ramesh R. Vetukuri (Lead / Corresponding author)

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)peer-review

1 Citation (Scopus)


RNA interference (RNAi) is a conserved cellular defense mechanism mediated by double-stranded RNA (dsRNA) that can regulate gene expression through targeted destruction of mRNAs (messenger RNAs). Recent studies have shown that spraying dsRNAs or small RNAs (sRNAs) that target essential genes of pathogens on plant surfaces can confer protection against pests and pathogens. Also called spray-induced gene silencing (SIGS), this strategy can be used for disease control and for transient gene silencing to study the function of genes in plant-pathogen interactions. Furthermore, as sRNAs can move locally, systemically, and cross-kingdom during plant-microbe interactions, SIGS allows quick detection and characterization of gene functions in pathogens and plants.

Original languageEnglish
Title of host publicationPlant Pathology
Subtitle of host publicationMethod and Protocols
EditorsNicola Luchi
Place of PublicationNew York
PublisherHumana Press
Number of pages16
ISBN (Electronic)9781071625170
ISBN (Print)9781071625163
Publication statusPublished - 2022

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Gene silencing
  • Oomycetes
  • Potato
  • RNAi
  • SIGS

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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