Spy vs. Spy: Selecting the best reporter for 19F NMR competition experiment

Guilherme Vieira de Castro, Alessio Ciulli (Lead / Corresponding author)

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Abstract

Systematic characterization of a series of fluorinated VHL ligands, varying binding affinity and position of the trifluoromethyl group, qualifies a spy molecule for competitive 19F NMR screening and reveals guiding principles to develop highly sensitive assays with low material consumption.

Original languageEnglish
Pages (from-to)1482-1485
Number of pages4
JournalChemical Communications
Volume55
Issue number10
Early online date15 Jan 2019
DOIs
Publication statusPublished - 4 Feb 2019

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Assays
Screening
Ligands
Nuclear magnetic resonance
Molecules
Experiments

Cite this

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abstract = "Systematic characterization of a series of fluorinated VHL ligands, varying binding affinity and position of the trifluoromethyl group, qualifies a spy molecule for competitive 19F NMR screening and reveals guiding principles to develop highly sensitive assays with low material consumption.",
author = "Castro, {Guilherme Vieira de} and Alessio Ciulli",
note = "This project has received funding from the European Research Council (ERC) under the European Union’s Seventh Frame- work Programme (FP7/2007−2013) as a Starting Grant to A.C. (grant agreement no. ERC-2012-StG-311460 DrugE3CRLs) and the Coordena{\cc}{\~a}o de Aperfei{\cc}oamento de Pessoal de N{\'i}vel Superior (CAPES, Ph.D. Studentship 7148-14-3 to G.V.C.). Biophysics and drug discovery activities were supported by Wellcome Trust strategic awards to Dundee (100476/Z/12/Z and 094090/Z/10/Z, respectively).",
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