Spy vs. Spy: Selecting the best reporter for 19F NMR competition experiment

Guilherme Vieira de Castro; Alessio Ciulli

doi:10.1039/C8CC09790A

Spy vs. Spy: Selecting the best reporter for 19F NMR competition experiment

Guilherme Vieira de Castro, Alessio Ciulli (Lead / Corresponding author)

Biological Chemistry and Drug Discovery

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

79 Downloads (Pure)

Abstract

Systematic characterization of a series of fluorinated VHL ligands, varying binding affinity and position of the trifluoromethyl group, qualifies a spy molecule for competitive ¹⁹F NMR screening and reveals guiding principles to develop highly sensitive assays with low material consumption.

Original language	English
Pages (from-to)	1482-1485
Number of pages	4
Journal	Chemical Communications
Volume	55
Issue number	10
Early online date	15 Jan 2019
DOIs	https://doi.org/10.1039/C8CC09790A
Publication status	Published - 4 Feb 2019

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10.1039/C8CC09790ALicence: CC BY

Final Published VersionFinal published version, 2.78 MBLicence: CC BY

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Fragment-based approaches to probe the surface of the VHL E3 ligase
Author: Castro, G., 2019
Supervisor: Ciulli, A. (Supervisor)
Student thesis: Doctoral Thesis › Doctor of Philosophy

Cite this

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title = "Spy vs. Spy: Selecting the best reporter for 19F NMR competition experiment",

abstract = "Systematic characterization of a series of fluorinated VHL ligands, varying binding affinity and position of the trifluoromethyl group, qualifies a spy molecule for competitive 19F NMR screening and reveals guiding principles to develop highly sensitive assays with low material consumption. ",

author = "Castro, {Guilherme Vieira de} and Alessio Ciulli",

note = "This project has received funding from the European Research Council (ERC) under the European Union{\textquoteright}s Seventh Frame- work Programme (FP7/2007−2013) as a Starting Grant to A.C. (grant agreement no. ERC-2012-StG-311460 DrugE3CRLs) and the Coordena{\c c}{\~a}o de Aperfei{\c c}oamento de Pessoal de N{\'i}vel Superior (CAPES, Ph.D. Studentship 7148-14-3 to G.V.C.). Biophysics and drug discovery activities were supported by Wellcome Trust strategic awards to Dundee (100476/Z/12/Z and 094090/Z/10/Z, respectively).",

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Spy vs. Spy: Selecting the best reporter for 19F NMR competition experiment

Abstract

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Fragment-based approaches to probe the surface of the VHL E3 ligase

Cite this