Stable-isotope labeling with amino acids in nematodes

Mark Larance, Aymeric P. Bailly, Ehsan Pourkarimi, Ronald T. Hay, Grant Buchanan, Sarah Coulthurst, Dimitris P. Xirodimas, Anton Gartner (Lead / Corresponding author), Angus I. Lamond (Lead / Corresponding author)

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    81 Citations (Scopus)

    Abstract

    We describe an approach for accurate quantitation of global protein dynamics in Caenorhabditis elegans. We adapted stable-isotope labeling with amino acids in cell culture (SILAC) for nematodes by feeding worms a heavy lysine- and heavy arginine-labeled Escherichia coli strain and report a genetic solution to elminate the problem of arginine-to-proline conversion. Combining our approach with quantitative proteomics methods, we characterized the heat-shock response in worms.

    Original languageEnglish
    Pages (from-to)849-851
    Number of pages3
    JournalNature Methods
    Volume8
    Issue number10
    DOIs
    Publication statusPublished - Oct 2011

    Keywords

    • QUANTITATIVE PROTEOMICS
    • CAENORHABDITIS-ELEGANS
    • PROTEIN QUANTIFICATION
    • CELL-CULTURE
    • SILAC
    • EXPRESSION

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