Stable-isotope labeling with amino acids in nematodes

Mark Larance; Aymeric P. Bailly; Ehsan Pourkarimi; Ronald T. Hay; Grant Buchanan; Sarah Coulthurst; Dimitris P. Xirodimas; Anton Gartner; Angus I. Lamond

doi:10.1038/NMETH.1679

Stable-isotope labeling with amino acids in nematodes

Mark Larance, Aymeric P. Bailly, Ehsan Pourkarimi, Ronald T. Hay, Grant Buchanan, Sarah Coulthurst, Dimitris P. Xirodimas, Anton Gartner (Lead / Corresponding author), Angus I. Lamond (Lead / Corresponding author)

Research output: Contribution to journal › Article › peer-review

97 Citations (Scopus)

Abstract

We describe an approach for accurate quantitation of global protein dynamics in Caenorhabditis elegans. We adapted stable-isotope labeling with amino acids in cell culture (SILAC) for nematodes by feeding worms a heavy lysine- and heavy arginine-labeled Escherichia coli strain and report a genetic solution to elminate the problem of arginine-to-proline conversion. Combining our approach with quantitative proteomics methods, we characterized the heat-shock response in worms.

Original language	English
Pages (from-to)	849-851
Number of pages	3
Journal	Nature Methods
Volume	8
Issue number	10
DOIs	https://doi.org/10.1038/NMETH.1679
Publication status	Published - Oct 2011

Keywords

QUANTITATIVE PROTEOMICS
CAENORHABDITIS-ELEGANS
PROTEIN QUANTIFICATION
CELL-CULTURE
SILAC
EXPRESSION

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10.1038/NMETH.1679

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@article{b00dda5bbc644f9988d0861323a1920d,

title = "Stable-isotope labeling with amino acids in nematodes",

abstract = "We describe an approach for accurate quantitation of global protein dynamics in Caenorhabditis elegans. We adapted stable-isotope labeling with amino acids in cell culture (SILAC) for nematodes by feeding worms a heavy lysine- and heavy arginine-labeled Escherichia coli strain and report a genetic solution to elminate the problem of arginine-to-proline conversion. Combining our approach with quantitative proteomics methods, we characterized the heat-shock response in worms.",

keywords = "QUANTITATIVE PROTEOMICS, CAENORHABDITIS-ELEGANS, PROTEIN QUANTIFICATION, CELL-CULTURE, SILAC, EXPRESSION",

author = "Mark Larance and Bailly, {Aymeric P.} and Ehsan Pourkarimi and Hay, {Ronald T.} and Grant Buchanan and Sarah Coulthurst and Xirodimas, {Dimitris P.} and Anton Gartner and Lamond, {Angus I.}",

year = "2011",

month = oct,

doi = "10.1038/NMETH.1679",

language = "English",

volume = "8",

pages = "849--851",

journal = "Nature Methods",

issn = "1548-7091",

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TY - JOUR

T1 - Stable-isotope labeling with amino acids in nematodes

AU - Larance, Mark

AU - Bailly, Aymeric P.

AU - Pourkarimi, Ehsan

AU - Hay, Ronald T.

AU - Buchanan, Grant

AU - Coulthurst, Sarah

AU - Xirodimas, Dimitris P.

AU - Gartner, Anton

AU - Lamond, Angus I.

PY - 2011/10

Y1 - 2011/10

N2 - We describe an approach for accurate quantitation of global protein dynamics in Caenorhabditis elegans. We adapted stable-isotope labeling with amino acids in cell culture (SILAC) for nematodes by feeding worms a heavy lysine- and heavy arginine-labeled Escherichia coli strain and report a genetic solution to elminate the problem of arginine-to-proline conversion. Combining our approach with quantitative proteomics methods, we characterized the heat-shock response in worms.

AB - We describe an approach for accurate quantitation of global protein dynamics in Caenorhabditis elegans. We adapted stable-isotope labeling with amino acids in cell culture (SILAC) for nematodes by feeding worms a heavy lysine- and heavy arginine-labeled Escherichia coli strain and report a genetic solution to elminate the problem of arginine-to-proline conversion. Combining our approach with quantitative proteomics methods, we characterized the heat-shock response in worms.

KW - QUANTITATIVE PROTEOMICS

KW - CAENORHABDITIS-ELEGANS

KW - PROTEIN QUANTIFICATION

KW - CELL-CULTURE

KW - SILAC

KW - EXPRESSION

UR - http://www.scopus.com/inward/record.url?scp=80053363595&partnerID=8YFLogxK

U2 - 10.1038/NMETH.1679

DO - 10.1038/NMETH.1679

M3 - Article

SN - 1548-7091

VL - 8

SP - 849

EP - 851

JO - Nature Methods

JF - Nature Methods

IS - 10

ER -

Stable-isotope labeling with amino acids in nematodes

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Keywords

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Combined Genetic and Biochemical Approaches to Uncover and Characterize Redundant Factors Involved in Late Stages of Recombinational Repair

Aref#d: 19770. Division of Gene Regulation and Expression Strategic Award

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Stable-isotope labeling with amino acids in nematodes

Abstract

Keywords

Access to Document

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Combined Genetic and Biochemical Approaches to Uncover and Characterize Redundant Factors Involved in Late Stages of Recombinational Repair

Aref#d: 19770. Division of Gene Regulation and Expression Strategic Award

Cite this