Stable-isotope labeling with amino acids in nematodes

TY - JOUR

T1 - Stable-isotope labeling with amino acids in nematodes

AU - Larance, Mark

AU - Bailly, Aymeric P.

AU - Pourkarimi, Ehsan

AU - Hay, Ronald T.

AU - Buchanan, Grant

AU - Coulthurst, Sarah

AU - Xirodimas, Dimitris P.

AU - Gartner, Anton

AU - Lamond, Angus I.

PY - 2011/10

Y1 - 2011/10

N2 - We describe an approach for accurate quantitation of global protein dynamics in Caenorhabditis elegans. We adapted stable-isotope labeling with amino acids in cell culture (SILAC) for nematodes by feeding worms a heavy lysine- and heavy arginine-labeled Escherichia coli strain and report a genetic solution to elminate the problem of arginine-to-proline conversion. Combining our approach with quantitative proteomics methods, we characterized the heat-shock response in worms.

AB - We describe an approach for accurate quantitation of global protein dynamics in Caenorhabditis elegans. We adapted stable-isotope labeling with amino acids in cell culture (SILAC) for nematodes by feeding worms a heavy lysine- and heavy arginine-labeled Escherichia coli strain and report a genetic solution to elminate the problem of arginine-to-proline conversion. Combining our approach with quantitative proteomics methods, we characterized the heat-shock response in worms.

KW - QUANTITATIVE PROTEOMICS

KW - CAENORHABDITIS-ELEGANS

KW - PROTEIN QUANTIFICATION

KW - CELL-CULTURE

KW - SILAC

KW - EXPRESSION

UR - https://www.scopus.com/pages/publications/80053363595

U2 - 10.1038/NMETH.1679

DO - 10.1038/NMETH.1679

M3 - Article

SN - 1548-7091

VL - 8

SP - 849

EP - 851

JO - Nature Methods

JF - Nature Methods

IS - 10

ER -