Stimulation of renal Na+ dicarboxylate cotransporter 1 by Na+/H+ exchanger regulating factor 2, serum and glucocorticoid inducible kinase isoforms, and protein kinase B

Christoph Boehmer, Hamdy M. Embark, Anna Bauer, Monica Palmada, Chris H. Yun, Edward J. Weinman, Hitoshi Endou, Philip Cohen, Sven Lahme, Karl Horst Bichler, Florian Lang

    Research output: Contribution to journalArticlepeer-review

    31 Citations (Scopus)

    Abstract

    Renal tubular citrate transport is accomplished by electrogenic Na + coupled dicarboxylate transporter NaDC-1, a carrier subjected to regulation by acidosis. Trafficking of the Na+/H+ exchanger NHE3 is controlled by NHE regulating factors NHERF-1 and NHERF-2 and the serum and glucocorticoid inducible kinase SGK1. To test for a possible involvement in NaDC-1 regulation, mRNA encoding NaDC-1 was injected into Xenopus oocytes with or without cRNA encoding NHERF-1, NHERF-2, SGK1, SGK2, SGK3, and/or the constitutively active form of the related protein kinase B (T308,S473DPKB). Succinate induced inward currents (I succ) were taken as a measure of transport rate. Coexpression of neither NHERF-1 nor NHERF-2 in NaDC-1 expressing oocytes significantly altered Isucc. On the other hand, coexpression of SGK1, SGK3, and T308,S473DPKB stimulated Isucc, an effect further stimulated by additional coexpression of NHERF-2 but not of NHERF-1. The action of the kinases and NHERF-2 may link urinary citrate excretion to proximal tubular H+ secretion.

    Original languageEnglish
    Pages (from-to)998-1003
    Number of pages6
    JournalBiochemical and Biophysical Research Communications
    Volume313
    Issue number4
    DOIs
    Publication statusPublished - 23 Jan 2004

    Keywords

    • Alkalosis
    • Citrate
    • Insulin
    • Kidney
    • Na/H exchanger
    • Proximal tubule
    • Urolithiasis

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