Stimulation of renal Na+ dicarboxylate cotransporter 1 by Na+/H+ exchanger regulating factor 2, serum and glucocorticoid inducible kinase isoforms, and protein kinase B

Christoph Boehmer; Hamdy M. Embark; Anna Bauer; Monica Palmada; Chris H. Yun; Edward J. Weinman; Hitoshi Endou; Philip Cohen; Sven Lahme; Karl Horst Bichler; Florian Lang

doi:10.1016/j.bbrc.2003.12.011

Stimulation of renal Na⁺ dicarboxylate cotransporter 1 by Na⁺/H⁺ exchanger regulating factor 2, serum and glucocorticoid inducible kinase isoforms, and protein kinase B

Christoph Boehmer
, Hamdy M. Embark
, Anna Bauer
, Monica Palmada
, Chris H. Yun
, Edward J. Weinman
, Hitoshi Endou
, Philip Cohen
, Sven Lahme
, Karl Horst Bichler
, Florian Lang

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Abstract

Renal tubular citrate transport is accomplished by electrogenic Na ⁺ coupled dicarboxylate transporter NaDC-1, a carrier subjected to regulation by acidosis. Trafficking of the Na⁺/H⁺ exchanger NHE3 is controlled by NHE regulating factors NHERF-1 and NHERF-2 and the serum and glucocorticoid inducible kinase SGK1. To test for a possible involvement in NaDC-1 regulation, mRNA encoding NaDC-1 was injected into Xenopus oocytes with or without cRNA encoding NHERF-1, NHERF-2, SGK1, SGK2, SGK3, and/or the constitutively active form of the related protein kinase B (^T308,S473DPKB). Succinate induced inward currents (I _succ) were taken as a measure of transport rate. Coexpression of neither NHERF-1 nor NHERF-2 in NaDC-1 expressing oocytes significantly altered I_succ. On the other hand, coexpression of SGK1, SGK3, and ^T308,S473DPKB stimulated I_succ, an effect further stimulated by additional coexpression of NHERF-2 but not of NHERF-1. The action of the kinases and NHERF-2 may link urinary citrate excretion to proximal tubular H⁺ secretion.

Original language	English
Pages (from-to)	998-1003
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	313
Issue number	4
DOIs	https://doi.org/10.1016/j.bbrc.2003.12.011
Publication status	Published - 23 Jan 2004

Keywords

Alkalosis
Citrate
Insulin
Kidney
Na/H exchanger
Proximal tubule
Urolithiasis

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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Cite this

Boehmer, C., Embark, H. M., Bauer, A., Palmada, M., Yun, C. H., Weinman, E. J., Endou, H., Cohen, P., Lahme, S., Bichler, K. H., & Lang, F. (2004). Stimulation of renal Na⁺ dicarboxylate cotransporter 1 by Na⁺/H⁺ exchanger regulating factor 2, serum and glucocorticoid inducible kinase isoforms, and protein kinase B. Biochemical and Biophysical Research Communications, 313(4), 998-1003. https://doi.org/10.1016/j.bbrc.2003.12.011

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title = "Stimulation of renal Na+ dicarboxylate cotransporter 1 by Na+/H+ exchanger regulating factor 2, serum and glucocorticoid inducible kinase isoforms, and protein kinase B",

abstract = "Renal tubular citrate transport is accomplished by electrogenic Na + coupled dicarboxylate transporter NaDC-1, a carrier subjected to regulation by acidosis. Trafficking of the Na+/H+ exchanger NHE3 is controlled by NHE regulating factors NHERF-1 and NHERF-2 and the serum and glucocorticoid inducible kinase SGK1. To test for a possible involvement in NaDC-1 regulation, mRNA encoding NaDC-1 was injected into Xenopus oocytes with or without cRNA encoding NHERF-1, NHERF-2, SGK1, SGK2, SGK3, and/or the constitutively active form of the related protein kinase B (T308,S473DPKB). Succinate induced inward currents (I succ) were taken as a measure of transport rate. Coexpression of neither NHERF-1 nor NHERF-2 in NaDC-1 expressing oocytes significantly altered Isucc. On the other hand, coexpression of SGK1, SGK3, and T308,S473DPKB stimulated Isucc, an effect further stimulated by additional coexpression of NHERF-2 but not of NHERF-1. The action of the kinases and NHERF-2 may link urinary citrate excretion to proximal tubular H+ secretion.",

keywords = "Alkalosis, Citrate, Insulin, Kidney, Na/H exchanger, Proximal tubule, Urolithiasis",

author = "Christoph Boehmer and Embark, \{Hamdy M.\} and Anna Bauer and Monica Palmada and Yun, \{Chris H.\} and Weinman, \{Edward J.\} and Hitoshi Endou and Philip Cohen and Sven Lahme and Bichler, \{Karl Horst\} and Florian Lang",

year = "2004",

month = jan,

day = "23",

doi = "10.1016/j.bbrc.2003.12.011",

language = "English",

volume = "313",

pages = "998--1003",

journal = "Biochemical and Biophysical Research Communications",

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Boehmer, C, Embark, HM, Bauer, A, Palmada, M, Yun, CH, Weinman, EJ, Endou, H, Cohen, P, Lahme, S, Bichler, KH & Lang, F 2004, 'Stimulation of renal Na⁺ dicarboxylate cotransporter 1 by Na⁺/H⁺ exchanger regulating factor 2, serum and glucocorticoid inducible kinase isoforms, and protein kinase B', Biochemical and Biophysical Research Communications, vol. 313, no. 4, pp. 998-1003. https://doi.org/10.1016/j.bbrc.2003.12.011

Stimulation of renal Na⁺ dicarboxylate cotransporter 1 by Na⁺/H⁺ exchanger regulating factor 2, serum and glucocorticoid inducible kinase isoforms, and protein kinase B. / Boehmer, Christoph; Embark, Hamdy M.; Bauer, Anna et al.
In: Biochemical and Biophysical Research Communications, Vol. 313, No. 4, 23.01.2004, p. 998-1003.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Stimulation of renal Na+ dicarboxylate cotransporter 1 by Na+/H+ exchanger regulating factor 2, serum and glucocorticoid inducible kinase isoforms, and protein kinase B

AU - Boehmer, Christoph

AU - Embark, Hamdy M.

AU - Bauer, Anna

AU - Palmada, Monica

AU - Yun, Chris H.

AU - Weinman, Edward J.

AU - Endou, Hitoshi

AU - Cohen, Philip

AU - Lahme, Sven

AU - Bichler, Karl Horst

AU - Lang, Florian

PY - 2004/1/23

Y1 - 2004/1/23

N2 - Renal tubular citrate transport is accomplished by electrogenic Na + coupled dicarboxylate transporter NaDC-1, a carrier subjected to regulation by acidosis. Trafficking of the Na+/H+ exchanger NHE3 is controlled by NHE regulating factors NHERF-1 and NHERF-2 and the serum and glucocorticoid inducible kinase SGK1. To test for a possible involvement in NaDC-1 regulation, mRNA encoding NaDC-1 was injected into Xenopus oocytes with or without cRNA encoding NHERF-1, NHERF-2, SGK1, SGK2, SGK3, and/or the constitutively active form of the related protein kinase B (T308,S473DPKB). Succinate induced inward currents (I succ) were taken as a measure of transport rate. Coexpression of neither NHERF-1 nor NHERF-2 in NaDC-1 expressing oocytes significantly altered Isucc. On the other hand, coexpression of SGK1, SGK3, and T308,S473DPKB stimulated Isucc, an effect further stimulated by additional coexpression of NHERF-2 but not of NHERF-1. The action of the kinases and NHERF-2 may link urinary citrate excretion to proximal tubular H+ secretion.

AB - Renal tubular citrate transport is accomplished by electrogenic Na + coupled dicarboxylate transporter NaDC-1, a carrier subjected to regulation by acidosis. Trafficking of the Na+/H+ exchanger NHE3 is controlled by NHE regulating factors NHERF-1 and NHERF-2 and the serum and glucocorticoid inducible kinase SGK1. To test for a possible involvement in NaDC-1 regulation, mRNA encoding NaDC-1 was injected into Xenopus oocytes with or without cRNA encoding NHERF-1, NHERF-2, SGK1, SGK2, SGK3, and/or the constitutively active form of the related protein kinase B (T308,S473DPKB). Succinate induced inward currents (I succ) were taken as a measure of transport rate. Coexpression of neither NHERF-1 nor NHERF-2 in NaDC-1 expressing oocytes significantly altered Isucc. On the other hand, coexpression of SGK1, SGK3, and T308,S473DPKB stimulated Isucc, an effect further stimulated by additional coexpression of NHERF-2 but not of NHERF-1. The action of the kinases and NHERF-2 may link urinary citrate excretion to proximal tubular H+ secretion.

KW - Alkalosis

KW - Citrate

KW - Insulin

KW - Kidney

KW - Na/H exchanger

KW - Proximal tubule

KW - Urolithiasis

UR - https://www.scopus.com/pages/publications/9144274968

U2 - 10.1016/j.bbrc.2003.12.011

DO - 10.1016/j.bbrc.2003.12.011

M3 - Article

C2 - 14706641

AN - SCOPUS:9144274968

SN - 0006-291X

VL - 313

SP - 998

EP - 1003

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 4

ER -