Stoichiometry and intracellular fate of TRIM-containing TCR complexes

TY - JOUR

T1 - Stoichiometry and intracellular fate of TRIM-containing TCR complexes

AU - Swamy, Mahima

AU - Siegers, Gabrielle M.

AU - Fiala, Gina J.

AU - Molnar, Eszter

AU - Dopfer, Elaine P.

AU - Fisch, Paul

AU - Schraven, Burkhart

AU - Schamel, Wolfgang W.A.

PY - 2010/3/18

Y1 - 2010/3/18

N2 - Background: Studying the stoichiometry and intracellular trafficking of the T cell antigen receptor (TCR) is pivotal in understanding its mechanisms of activation. The αβTCR includes the antigen-binding TCRαβ heterodimer as well as the signal transducing CD3εγ, CD3εδ and ζ2subunits. Although the TCR-interacting molecule (TRIM) is also part of the αβTCR complex, it has not been included in most reports so far. Results: We used the native antibody-based mobility shift (NAMOS) assay in a first dimension (1D) blue native (BN)-PAGE and a 2D BN-/BN-PAGE to demonstrate that the stoichiometry of the digitonin-solublized TRIM-containing αβTCR is TCRαβCD3ε2γ δζ2TRIM2. Smaller αβTCR complexes possess a TCRαβ CD3ε2γδζ 2stoichiometry. Complexes of these sizes were detected in T cell lines as well as in primary human and mouse T cells. Stimulating the αβTCR with anti-CD3 antibodies, we demonstrate by confocal laser scanning microscopy that CD3ε colocalizes with ζ and both are degraded upon prolonged stimulation, possibly within the lysosomal compartment. In contrast, a substantial fraction of TRIM does not colocalize with ζ. Furthermore, TRIM neither moves to lysosomes nor is degraded. Immunoprecipitation studies and BN-PAGE indicate that TRIM also associates with the γδTCR. Conclusions: Small αβTCR complexes have a TCRαβ CD3ε2γδζ 2stoichiometry; whereas those associated with one TRIM dimer are TCRαβ CD3ε2γδζ2TRIM 2. TRIM is differentially processed compared to CD3 and ζ subunits after T cell activation and is not degraded. The γδTCR also associates with TRIM.

AB - Background: Studying the stoichiometry and intracellular trafficking of the T cell antigen receptor (TCR) is pivotal in understanding its mechanisms of activation. The αβTCR includes the antigen-binding TCRαβ heterodimer as well as the signal transducing CD3εγ, CD3εδ and ζ2subunits. Although the TCR-interacting molecule (TRIM) is also part of the αβTCR complex, it has not been included in most reports so far. Results: We used the native antibody-based mobility shift (NAMOS) assay in a first dimension (1D) blue native (BN)-PAGE and a 2D BN-/BN-PAGE to demonstrate that the stoichiometry of the digitonin-solublized TRIM-containing αβTCR is TCRαβCD3ε2γ δζ2TRIM2. Smaller αβTCR complexes possess a TCRαβ CD3ε2γδζ 2stoichiometry. Complexes of these sizes were detected in T cell lines as well as in primary human and mouse T cells. Stimulating the αβTCR with anti-CD3 antibodies, we demonstrate by confocal laser scanning microscopy that CD3ε colocalizes with ζ and both are degraded upon prolonged stimulation, possibly within the lysosomal compartment. In contrast, a substantial fraction of TRIM does not colocalize with ζ. Furthermore, TRIM neither moves to lysosomes nor is degraded. Immunoprecipitation studies and BN-PAGE indicate that TRIM also associates with the γδTCR. Conclusions: Small αβTCR complexes have a TCRαβ CD3ε2γδζ 2stoichiometry; whereas those associated with one TRIM dimer are TCRαβ CD3ε2γδζ2TRIM 2. TRIM is differentially processed compared to CD3 and ζ subunits after T cell activation and is not degraded. The γδTCR also associates with TRIM.

UR - https://www.scopus.com/pages/publications/78649861313

U2 - 10.1186/1478-811X-8-5

DO - 10.1186/1478-811X-8-5

M3 - Review article

AN - SCOPUS:78649861313

SN - 1478-811X

VL - 8

SP - 1

EP - 10

JO - Cell Communication and Signaling

JF - Cell Communication and Signaling

M1 - 5

ER -