Stomatal vs. genome size in angiosperms

J. G. Hodgson, M. Sharafi, A. Jalili, S. Diaz, G. Montserrat-Marti, C. Palmer, B. Cerabolini, S. Pierce, B. Hamzehee, Y. Asri, Z. Jamzad, P. Wilson, J. A. Raven, S. R. Band, S. Basconcelo, A. Bogard, G. Carter, M. Charles, P. Castro-Diez, J. H. C. Cornelissen & 22 others G. Funes, G. Jones, M. Khoshnevis, N. Perez-Harguindeguy, M. C. Perez-Rontome, F. A. Shirvany, F. Vendramini, S. Yazdani, R. Abbas-Azimi, S. Boustani, M. Dehghan, J. Guerrero-Campo, A. Hynd, E. Kowsary, F. Kazemi-Saeed, B. Siavash, P. Villar-Salvador, R. Craigie, A. Naqinezhad, A. Romo-Diez, L. de Torres Espuny, E. Simmons

    Research output: Contribution to journalArticle

    85 Citations (Scopus)

    Abstract

    Background and Aims Genome size is a function, and the product, of cell volume. As such it is contingent on ecological circumstance. The nature of 'this ecological circumstance' is, however, hotly debated. Here, we investigate for angiosperms whether stomatal size may be this 'missing link': the primary determinant of genome size. Stomata are crucial for photosynthesis and their size affects functional efficiency.

    Methods Stomatal and leaf characteristics were measured for 1442 species from Argentina, Iran, Spain and the UK and, using PCA, some emergent ecological and taxonomic patterns identified. Subsequently, an assessment of the relationship between genome-size values obtained from the Plant DNA C-values database and measurements of stomatal size was carried out.

    Key Results Stomatal size is an ecologically important attribute. It varies with life-history (woody species, herbaceous species < vernal geophytes) and contributes to ecologically and physiologically important axes of leaf specialization. Moreover, it is positively correlated with genome size across a wide range of major taxa.

    Conclusions Stomatal size predicts genome size within angiosperms. Correlation is not, however, proof of causality and here our interpretation is hampered by unexpected deficiencies in the scientific literature. Firstly, there are discrepancies between our own observations and established ideas about the ecological significance of stomatal size; very large stomata, theoretically facilitating photosynthesis in deep shade, were, in this study (and in other studies), primarily associated with vernal geophytes of unshaded habitats. Secondly, the lower size limit at which stomata can function efficiently, and the ecological circumstances under which these minute stomata might occur, have not been satisfactorally resolved. Thus, our hypothesis, that the optimization of stomatal size for functional efficiency is a major ecological determinant of genome size, remains unproven.

    Original languageEnglish
    Pages (from-to)573-584
    Number of pages12
    JournalAnnals of Botany
    Volume105
    Issue number4
    DOIs
    Publication statusPublished - 2010

    Keywords

    • Stomatal size
    • genome size
    • seed size
    • life history
    • photosynthesis
    • allometry
    • ecology
    • evolution
    • SLA
    • leaf structure
    • CAM
    • C-4
    • NUCLEAR-DNA CONTENT
    • RELATIVE GROWTH-RATE
    • FLOWERING PLANTS
    • LINEAR SEQUENCE
    • SELFISH DNA
    • SEED SIZE
    • CELL-SIZE
    • LEAF-AREA
    • APG III
    • EVOLUTION

    Cite this

    Hodgson, J. G., Sharafi, M., Jalili, A., Diaz, S., Montserrat-Marti, G., Palmer, C., ... Simmons, E. (2010). Stomatal vs. genome size in angiosperms. Annals of Botany, 105(4), 573-584. https://doi.org/10.1093/aob/mcq011
    Hodgson, J. G. ; Sharafi, M. ; Jalili, A. ; Diaz, S. ; Montserrat-Marti, G. ; Palmer, C. ; Cerabolini, B. ; Pierce, S. ; Hamzehee, B. ; Asri, Y. ; Jamzad, Z. ; Wilson, P. ; Raven, J. A. ; Band, S. R. ; Basconcelo, S. ; Bogard, A. ; Carter, G. ; Charles, M. ; Castro-Diez, P. ; Cornelissen, J. H. C. ; Funes, G. ; Jones, G. ; Khoshnevis, M. ; Perez-Harguindeguy, N. ; Perez-Rontome, M. C. ; Shirvany, F. A. ; Vendramini, F. ; Yazdani, S. ; Abbas-Azimi, R. ; Boustani, S. ; Dehghan, M. ; Guerrero-Campo, J. ; Hynd, A. ; Kowsary, E. ; Kazemi-Saeed, F. ; Siavash, B. ; Villar-Salvador, P. ; Craigie, R. ; Naqinezhad, A. ; Romo-Diez, A. ; de Torres Espuny, L. ; Simmons, E. / Stomatal vs. genome size in angiosperms. In: Annals of Botany. 2010 ; Vol. 105, No. 4. pp. 573-584.
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    abstract = "Background and Aims Genome size is a function, and the product, of cell volume. As such it is contingent on ecological circumstance. The nature of 'this ecological circumstance' is, however, hotly debated. Here, we investigate for angiosperms whether stomatal size may be this 'missing link': the primary determinant of genome size. Stomata are crucial for photosynthesis and their size affects functional efficiency.Methods Stomatal and leaf characteristics were measured for 1442 species from Argentina, Iran, Spain and the UK and, using PCA, some emergent ecological and taxonomic patterns identified. Subsequently, an assessment of the relationship between genome-size values obtained from the Plant DNA C-values database and measurements of stomatal size was carried out.Key Results Stomatal size is an ecologically important attribute. It varies with life-history (woody species, herbaceous species < vernal geophytes) and contributes to ecologically and physiologically important axes of leaf specialization. Moreover, it is positively correlated with genome size across a wide range of major taxa.Conclusions Stomatal size predicts genome size within angiosperms. Correlation is not, however, proof of causality and here our interpretation is hampered by unexpected deficiencies in the scientific literature. Firstly, there are discrepancies between our own observations and established ideas about the ecological significance of stomatal size; very large stomata, theoretically facilitating photosynthesis in deep shade, were, in this study (and in other studies), primarily associated with vernal geophytes of unshaded habitats. Secondly, the lower size limit at which stomata can function efficiently, and the ecological circumstances under which these minute stomata might occur, have not been satisfactorally resolved. Thus, our hypothesis, that the optimization of stomatal size for functional efficiency is a major ecological determinant of genome size, remains unproven.",
    keywords = "Stomatal size, genome size, seed size, life history, photosynthesis, allometry, ecology, evolution, SLA, leaf structure, CAM, C-4, NUCLEAR-DNA CONTENT, RELATIVE GROWTH-RATE, FLOWERING PLANTS, LINEAR SEQUENCE, SELFISH DNA, SEED SIZE, CELL-SIZE, LEAF-AREA, APG III, EVOLUTION",
    author = "Hodgson, {J. G.} and M. Sharafi and A. Jalili and S. Diaz and G. Montserrat-Marti and C. Palmer and B. Cerabolini and S. Pierce and B. Hamzehee and Y. Asri and Z. Jamzad and P. Wilson and Raven, {J. A.} and Band, {S. R.} and S. Basconcelo and A. Bogard and G. Carter and M. Charles and P. Castro-Diez and Cornelissen, {J. H. C.} and G. Funes and G. Jones and M. Khoshnevis and N. Perez-Harguindeguy and Perez-Rontome, {M. C.} and Shirvany, {F. A.} and F. Vendramini and S. Yazdani and R. Abbas-Azimi and S. Boustani and M. Dehghan and J. Guerrero-Campo and A. Hynd and E. Kowsary and F. Kazemi-Saeed and B. Siavash and P. Villar-Salvador and R. Craigie and A. Naqinezhad and A. Romo-Diez and {de Torres Espuny}, L. and E. Simmons",
    year = "2010",
    doi = "10.1093/aob/mcq011",
    language = "English",
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    pages = "573--584",
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    Hodgson, JG, Sharafi, M, Jalili, A, Diaz, S, Montserrat-Marti, G, Palmer, C, Cerabolini, B, Pierce, S, Hamzehee, B, Asri, Y, Jamzad, Z, Wilson, P, Raven, JA, Band, SR, Basconcelo, S, Bogard, A, Carter, G, Charles, M, Castro-Diez, P, Cornelissen, JHC, Funes, G, Jones, G, Khoshnevis, M, Perez-Harguindeguy, N, Perez-Rontome, MC, Shirvany, FA, Vendramini, F, Yazdani, S, Abbas-Azimi, R, Boustani, S, Dehghan, M, Guerrero-Campo, J, Hynd, A, Kowsary, E, Kazemi-Saeed, F, Siavash, B, Villar-Salvador, P, Craigie, R, Naqinezhad, A, Romo-Diez, A, de Torres Espuny, L & Simmons, E 2010, 'Stomatal vs. genome size in angiosperms', Annals of Botany, vol. 105, no. 4, pp. 573-584. https://doi.org/10.1093/aob/mcq011

    Stomatal vs. genome size in angiosperms. / Hodgson, J. G.; Sharafi, M.; Jalili, A.; Diaz, S.; Montserrat-Marti, G.; Palmer, C.; Cerabolini, B.; Pierce, S.; Hamzehee, B.; Asri, Y.; Jamzad, Z.; Wilson, P.; Raven, J. A.; Band, S. R.; Basconcelo, S.; Bogard, A.; Carter, G.; Charles, M.; Castro-Diez, P.; Cornelissen, J. H. C.; Funes, G.; Jones, G.; Khoshnevis, M.; Perez-Harguindeguy, N.; Perez-Rontome, M. C.; Shirvany, F. A.; Vendramini, F.; Yazdani, S.; Abbas-Azimi, R.; Boustani, S.; Dehghan, M.; Guerrero-Campo, J.; Hynd, A.; Kowsary, E.; Kazemi-Saeed, F.; Siavash, B.; Villar-Salvador, P.; Craigie, R.; Naqinezhad, A.; Romo-Diez, A.; de Torres Espuny, L.; Simmons, E.

    In: Annals of Botany, Vol. 105, No. 4, 2010, p. 573-584.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Stomatal vs. genome size in angiosperms

    AU - Hodgson, J. G.

    AU - Sharafi, M.

    AU - Jalili, A.

    AU - Diaz, S.

    AU - Montserrat-Marti, G.

    AU - Palmer, C.

    AU - Cerabolini, B.

    AU - Pierce, S.

    AU - Hamzehee, B.

    AU - Asri, Y.

    AU - Jamzad, Z.

    AU - Wilson, P.

    AU - Raven, J. A.

    AU - Band, S. R.

    AU - Basconcelo, S.

    AU - Bogard, A.

    AU - Carter, G.

    AU - Charles, M.

    AU - Castro-Diez, P.

    AU - Cornelissen, J. H. C.

    AU - Funes, G.

    AU - Jones, G.

    AU - Khoshnevis, M.

    AU - Perez-Harguindeguy, N.

    AU - Perez-Rontome, M. C.

    AU - Shirvany, F. A.

    AU - Vendramini, F.

    AU - Yazdani, S.

    AU - Abbas-Azimi, R.

    AU - Boustani, S.

    AU - Dehghan, M.

    AU - Guerrero-Campo, J.

    AU - Hynd, A.

    AU - Kowsary, E.

    AU - Kazemi-Saeed, F.

    AU - Siavash, B.

    AU - Villar-Salvador, P.

    AU - Craigie, R.

    AU - Naqinezhad, A.

    AU - Romo-Diez, A.

    AU - de Torres Espuny, L.

    AU - Simmons, E.

    PY - 2010

    Y1 - 2010

    N2 - Background and Aims Genome size is a function, and the product, of cell volume. As such it is contingent on ecological circumstance. The nature of 'this ecological circumstance' is, however, hotly debated. Here, we investigate for angiosperms whether stomatal size may be this 'missing link': the primary determinant of genome size. Stomata are crucial for photosynthesis and their size affects functional efficiency.Methods Stomatal and leaf characteristics were measured for 1442 species from Argentina, Iran, Spain and the UK and, using PCA, some emergent ecological and taxonomic patterns identified. Subsequently, an assessment of the relationship between genome-size values obtained from the Plant DNA C-values database and measurements of stomatal size was carried out.Key Results Stomatal size is an ecologically important attribute. It varies with life-history (woody species, herbaceous species < vernal geophytes) and contributes to ecologically and physiologically important axes of leaf specialization. Moreover, it is positively correlated with genome size across a wide range of major taxa.Conclusions Stomatal size predicts genome size within angiosperms. Correlation is not, however, proof of causality and here our interpretation is hampered by unexpected deficiencies in the scientific literature. Firstly, there are discrepancies between our own observations and established ideas about the ecological significance of stomatal size; very large stomata, theoretically facilitating photosynthesis in deep shade, were, in this study (and in other studies), primarily associated with vernal geophytes of unshaded habitats. Secondly, the lower size limit at which stomata can function efficiently, and the ecological circumstances under which these minute stomata might occur, have not been satisfactorally resolved. Thus, our hypothesis, that the optimization of stomatal size for functional efficiency is a major ecological determinant of genome size, remains unproven.

    AB - Background and Aims Genome size is a function, and the product, of cell volume. As such it is contingent on ecological circumstance. The nature of 'this ecological circumstance' is, however, hotly debated. Here, we investigate for angiosperms whether stomatal size may be this 'missing link': the primary determinant of genome size. Stomata are crucial for photosynthesis and their size affects functional efficiency.Methods Stomatal and leaf characteristics were measured for 1442 species from Argentina, Iran, Spain and the UK and, using PCA, some emergent ecological and taxonomic patterns identified. Subsequently, an assessment of the relationship between genome-size values obtained from the Plant DNA C-values database and measurements of stomatal size was carried out.Key Results Stomatal size is an ecologically important attribute. It varies with life-history (woody species, herbaceous species < vernal geophytes) and contributes to ecologically and physiologically important axes of leaf specialization. Moreover, it is positively correlated with genome size across a wide range of major taxa.Conclusions Stomatal size predicts genome size within angiosperms. Correlation is not, however, proof of causality and here our interpretation is hampered by unexpected deficiencies in the scientific literature. Firstly, there are discrepancies between our own observations and established ideas about the ecological significance of stomatal size; very large stomata, theoretically facilitating photosynthesis in deep shade, were, in this study (and in other studies), primarily associated with vernal geophytes of unshaded habitats. Secondly, the lower size limit at which stomata can function efficiently, and the ecological circumstances under which these minute stomata might occur, have not been satisfactorally resolved. Thus, our hypothesis, that the optimization of stomatal size for functional efficiency is a major ecological determinant of genome size, remains unproven.

    KW - Stomatal size

    KW - genome size

    KW - seed size

    KW - life history

    KW - photosynthesis

    KW - allometry

    KW - ecology

    KW - evolution

    KW - SLA

    KW - leaf structure

    KW - CAM

    KW - C-4

    KW - NUCLEAR-DNA CONTENT

    KW - RELATIVE GROWTH-RATE

    KW - FLOWERING PLANTS

    KW - LINEAR SEQUENCE

    KW - SELFISH DNA

    KW - SEED SIZE

    KW - CELL-SIZE

    KW - LEAF-AREA

    KW - APG III

    KW - EVOLUTION

    U2 - 10.1093/aob/mcq011

    DO - 10.1093/aob/mcq011

    M3 - Article

    VL - 105

    SP - 573

    EP - 584

    JO - Annals of Botany

    JF - Annals of Botany

    SN - 0305-7364

    IS - 4

    ER -

    Hodgson JG, Sharafi M, Jalili A, Diaz S, Montserrat-Marti G, Palmer C et al. Stomatal vs. genome size in angiosperms. Annals of Botany. 2010;105(4):573-584. https://doi.org/10.1093/aob/mcq011