Structural and biochemical characterization of the exopolysaccharide deacetylase Agd3 required for Aspergillus fumigatus biofilm formation

Natalie C. Bamford, François Le Mauff, Jaime C. Van Loon, Hanna Ostapska, Brendan D. Snarr, Yongzhen Zhang, Elena N. Kitova, John S. Klassen, Jeroen D. C. Codée, Donald C. Sheppard (Lead / Corresponding author), P. Lynne Howell (Lead / Corresponding author)

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Abstract

The exopolysaccharide galactosaminogalactan (GAG) is an important virulence factor of the fungal pathogen Aspergillus fumigatus. Deletion of a gene encoding a putative deacetylase, Agd3, leads to defects in GAG deacetylation, biofilm formation, and virulence. Here, we show that Agd3 deacetylates GAG in a metal-dependent manner, and is the founding member of carbohydrate esterase family CE18. The active site is formed by four catalytic motifs that are essential for activity. The structure of Agd3 includes an elongated substrate-binding cleft formed by a carbohydrate binding module (CBM) that is the founding member of CBM family 87. Agd3 homologues are encoded in previously unidentified putative bacterial exopolysaccharide biosynthetic operons and in other fungal genomes.

Original languageEnglish
Article number2450
Number of pages13
JournalNature Communications
Volume11
DOIs
Publication statusPublished - 15 May 2020

Keywords

  • Biofilms
  • Fungi
  • Glycobiology
  • X-ray crystallography

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    Bamford, N. C., Le Mauff, F., Van Loon, J. C., Ostapska, H., Snarr, B. D., Zhang, Y., Kitova, E. N., Klassen, J. S., Codée, J. D. C., Sheppard, D. C., & Howell, P. L. (2020). Structural and biochemical characterization of the exopolysaccharide deacetylase Agd3 required for Aspergillus fumigatus biofilm formation. Nature Communications, 11, [2450]. https://doi.org/10.1038/s41467-020-16144-5