Projects per year
Abstract
The small ubiquitin-like modifier (SUMO) can form polymeric chains that are important signals in cellular processes such as meiosis, genome maintenance and stress response. The SUMO-targeted ubiquitin ligase RNF4 engages with SUMO chains on linked substrates and catalyses their ubiquitination, which targets substrates for proteasomal degradation. Here we use a segmental labelling approach combined with solution nuclear magnetic resonance (NMR) spectroscopy and biochemical characterization to reveal how RNF4 manipulates the conformation of the SUMO chain, thereby facilitating optimal delivery of the distal SUMO domain for ubiquitin transfer.
Original language | English |
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Article number | 4217 |
Number of pages | 12 |
Journal | Nature Communications |
Volume | 5 |
DOIs | |
Publication status | Published - 27 Jun 2014 |
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- 3 Finished
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Strategic Award: Wellcome Trust Technology Platform
Blow, J. (Investigator), Lamond, A. (Investigator) & Owen-Hughes, T. (Investigator)
1/01/13 → 30/09/18
Project: Research
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Mechanism of Poly-SUMO Chain Recognition by the Ubiquitin Ligase RNF4 (joint with Imperial College London)
Hay, R. (Investigator)
Biotechnology and Biological Sciences Research Council
21/12/12 → 20/01/16
Project: Research
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Determining the Role and Mechanism of Action of SUMO Targeted Ubiquitin Ligase RNF4 in Maintaining Genome Integrity (Senior Investigator Award)
Hay, R. (Investigator)
1/10/12 → 31/01/20
Project: Research