Structural insights into electron transfer in caa3 -type cytochrome oxidase

Joseph A. Lyons, David Aragão, Orla Slattery, Andrei V. Pisliakov, Tewfik Soulimane (Lead / Corresponding author), Martin Caffrey (Lead / Corresponding author)

    Research output: Contribution to journalLetter

    81 Citations (Scopus)

    Abstract

    Cytochrome c oxidase is a member of the haem copper oxidase superfamily (HCO). HCOs function as the terminal enzymes in the respiratory chain of mitochondria and aerobic prokaryotes, coupling molecular oxygen reduction to transmembrane proton pumping. Integral to the enzyme's function is the transfer of electrons from cytochrome c to the oxidase via a transient association of the two proteins. Electron entry and exit are proposed to occur from the same site on cytochrome c. Here we report the crystal structure of the caa 3-type cytochrome oxidase from Thermus thermophilus, which has a covalently tethered cytochrome c domain. Crystals were grown in a bicontinuous mesophase using a synthetic short-chain monoacylglycerol as the hosting lipid. From the electron density map, at 2.36 Å resolution, a novel integral membrane subunit and a native glycoglycerophospholipid embedded in the complex were identified. Contrary to previous electron transfer mechanisms observed for soluble cytochrome c, the structure reveals the architecture of the electron transfer complex for the fused cupredoxin/cytochrome c domain, which implicates different sites on cytochrome c for electron entry and exit. Support for an alternative to the classical proton gate characteristic of this HCO class is presented.
    Original languageEnglish
    Pages (from-to)514-518
    Number of pages5
    JournalNature
    Volume487
    Issue number7408
    DOIs
    Publication statusPublished - 26 Jul 2012

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    Lyons, J. A., Aragão, D., Slattery, O., Pisliakov, A. V., Soulimane, T., & Caffrey, M. (2012). Structural insights into electron transfer in caa3 -type cytochrome oxidase. Nature, 487(7408), 514-518. https://doi.org/10.1038/nature11182