Structural studies on a lipoarabinogalactan of Crithidia fasciculata

Pascal  Schneider; Achim Treumann; Kenneth G.  Milne; Malcolm J.  McConville; Nicole  Zitzmann; Michael A. J.  Ferguson

Structural studies on a lipoarabinogalactan of Crithidia fasciculata

Pascal Schneider
, Achim Treumann
, Kenneth G. Milne
, Malcolm J. McConville
, Nicole Zitzmann
, Michael A. J. Ferguson

Research output: Contribution to journal › Article › peer-review

Abstract

The monosaccharide D-arabinopyranose has only been found in glycoconjugates of the trypanosomatid parasites Leishmania major, Endotrypanum schaudinni and Crithidia fasciculata. The donor molecule for the relevant arabinosyltransferases is known to be GDP-a-D-Arap in L. major and C. fasciculata, and the latter organism is being used to study the biosynthesis of GDP-a-D-Arap. In this study, we describe the structure of the terminal product of arabinose metabolism in C. fasciculata, namely lipoarabinogalactan. This molecule was purified by hydrophobic-interaction chromatography and studied by a variety of techniques, including gas chromatography-mass spectrometry, electrospray mass spectrometry and chemical and enzymic digestions. These data show that lipoarabinogalactan contains a previously described D-arabino-D-galactan polysaccharide component covalently attached to a glycosylphosphatidylinositol type of membrane anchor that is similar to, but not identical with, that found in the lipophosphoglycans of the Leishmania.

Original language	English
Pages (from-to)	963-971
Number of pages	9
Journal	Biochemical Journal
Volume	313
Issue number	3
Publication status	Published - 1 Feb 1996

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@article{5eb0e68037f24ce7b04a7a8edfea26b3,

title = "Structural studies on a lipoarabinogalactan of Crithidia fasciculata",

abstract = "The monosaccharide D-arabinopyranose has only been found in glycoconjugates of the trypanosomatid parasites Leishmania major, Endotrypanum schaudinni and Crithidia fasciculata. The donor molecule for the relevant arabinosyltransferases is known to be GDP-a-D-Arap in L. major and C. fasciculata, and the latter organism is being used to study the biosynthesis of GDP-a-D-Arap. In this study, we describe the structure of the terminal product of arabinose metabolism in C. fasciculata, namely lipoarabinogalactan. This molecule was purified by hydrophobic-interaction chromatography and studied by a variety of techniques, including gas chromatography-mass spectrometry, electrospray mass spectrometry and chemical and enzymic digestions. These data show that lipoarabinogalactan contains a previously described D-arabino-D-galactan polysaccharide component covalently attached to a glycosylphosphatidylinositol type of membrane anchor that is similar to, but not identical with, that found in the lipophosphoglycans of the Leishmania.",

author = "Pascal Schneider and Achim Treumann and Milne, \{Kenneth G.\} and McConville, \{Malcolm J.\} and Nicole Zitzmann and Ferguson, \{Michael A. J.\}",

year = "1996",

month = feb,

day = "1",

language = "English",

volume = "313",

pages = "963--971",

journal = "Biochemical Journal",

issn = "0264-6021",

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TY - JOUR

T1 - Structural studies on a lipoarabinogalactan of Crithidia fasciculata

AU - Schneider, Pascal

AU - Treumann, Achim

AU - Milne, Kenneth G.

AU - McConville, Malcolm J.

AU - Zitzmann, Nicole

AU - Ferguson, Michael A. J.

PY - 1996/2/1

Y1 - 1996/2/1

N2 - The monosaccharide D-arabinopyranose has only been found in glycoconjugates of the trypanosomatid parasites Leishmania major, Endotrypanum schaudinni and Crithidia fasciculata. The donor molecule for the relevant arabinosyltransferases is known to be GDP-a-D-Arap in L. major and C. fasciculata, and the latter organism is being used to study the biosynthesis of GDP-a-D-Arap. In this study, we describe the structure of the terminal product of arabinose metabolism in C. fasciculata, namely lipoarabinogalactan. This molecule was purified by hydrophobic-interaction chromatography and studied by a variety of techniques, including gas chromatography-mass spectrometry, electrospray mass spectrometry and chemical and enzymic digestions. These data show that lipoarabinogalactan contains a previously described D-arabino-D-galactan polysaccharide component covalently attached to a glycosylphosphatidylinositol type of membrane anchor that is similar to, but not identical with, that found in the lipophosphoglycans of the Leishmania.

AB - The monosaccharide D-arabinopyranose has only been found in glycoconjugates of the trypanosomatid parasites Leishmania major, Endotrypanum schaudinni and Crithidia fasciculata. The donor molecule for the relevant arabinosyltransferases is known to be GDP-a-D-Arap in L. major and C. fasciculata, and the latter organism is being used to study the biosynthesis of GDP-a-D-Arap. In this study, we describe the structure of the terminal product of arabinose metabolism in C. fasciculata, namely lipoarabinogalactan. This molecule was purified by hydrophobic-interaction chromatography and studied by a variety of techniques, including gas chromatography-mass spectrometry, electrospray mass spectrometry and chemical and enzymic digestions. These data show that lipoarabinogalactan contains a previously described D-arabino-D-galactan polysaccharide component covalently attached to a glycosylphosphatidylinositol type of membrane anchor that is similar to, but not identical with, that found in the lipophosphoglycans of the Leishmania.

M3 - Article

SN - 0264-6021

VL - 313

SP - 963

EP - 971

JO - Biochemical Journal

JF - Biochemical Journal

IS - 3

ER -

Structural studies on a lipoarabinogalactan of Crithidia fasciculata

Abstract

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