Structural studies on a lipoarabinogalactan of Crithidia fasciculata

Pascal Schneider, Achim Treumann, Kenneth G. Milne, Malcolm J. McConville, Nicole Zitzmann, Michael A. J. Ferguson

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    23 Citations (Scopus)

    Abstract

    The monosaccharide D-arabinopyranose has only been found in glycoconjugates of the trypanosomatid parasites Leishmania major, Endotrypanum schaudinni and Crithidia fasciculata. The donor molecule for the relevant arabinosyltransferases is known to be GDP-a-D-Arap in L. major and C. fasciculata, and the latter organism is being used to study the biosynthesis of GDP-a-D-Arap. In this study, we describe the structure of the terminal product of arabinose metabolism in C. fasciculata, namely lipoarabinogalactan. This molecule was purified by hydrophobic-interaction chromatography and studied by a variety of techniques, including gas chromatography-mass spectrometry, electrospray mass spectrometry and chemical and enzymic digestions. These data show that lipoarabinogalactan contains a previously described D-arabino-D-galactan polysaccharide component covalently attached to a glycosylphosphatidylinositol type of membrane anchor that is similar to, but not identical with, that found in the lipophosphoglycans of the Leishmania.

    Original languageEnglish
    Pages (from-to)963-971
    Number of pages9
    JournalBiochemical Journal
    Volume313
    Issue number3
    Publication statusPublished - 1 Feb 1996

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