Structural studies on the polar glycoinositol phospholipids of Trypanosoma (Schizotrypanum) dionisii from bats

Marta H. Branquinha, Alane B. Vermelho, Igor C. Almeida, Angela Mehlert, Michael A. J. Ferguson

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    Abstract

    The polar glycoinositol phospholipids (GIPLs) of a Trypanosoma species that belongs to the Schizotrypanum subgenus were purified by reversed-phase and normal-phase liquid chromatography and analysed by negative-ion mode electrospray-mass spectrometry (ES-MS). The phosphatidylinositol moieties were released by nitrous acid deamination and identified as ceramide- and alkylacylglycerol-containing species. The structures of the GIPLs were determined using chemical treatments, sequential exoglycosidase digestions and positive-ion mode ES-MS-MS. All of the GIPLs were based on the same Man alpha 1-2Man alpha 1-2Man alpha 1-6Man alpha 1-4(NH2-CH2CH2-HPO3-)GlcN-PI core with single terminal Galf residue substitutions either on the terminal nonreducing Man or on the second alpha Man residue from the inositol and with either ethanolamine phosphate or 2-aminoethylphosphonate on the third alpha Man residue from the inositol. The T. (S.) dionisii GIPLs are compared with those of T. (S.) cruzi, a closely related species of the Schisotrypanum subgenus. (C) 1999 Elsevier Science B.V. All rights reserved.

    Original languageEnglish
    Pages (from-to)179-189
    Number of pages11
    JournalMolecular and Biochemical Parasitology
    Volume102
    Issue number1
    DOIs
    Publication statusPublished - 30 Jul 1999

    Keywords

    • Electrospray
    • Glycoinositol phospholipids
    • Glycosylphosphatidylinositol (GPI)
    • Inositol-phosphoceramide
    • Schizotrypanum
    • Trypanosomatidae

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