Structure and function of the bacterial root microbiota in wild and domesticated barley

TY - JOUR

T1 - Structure and function of the bacterial root microbiota in wild and domesticated barley

AU - Bulgarelli, Davide

AU - Garrido-Oter, Ruben

AU - Münch, Philipp C.

AU - Weiman, Aaron

AU - Dröge, Johannes

AU - Pan, Yao

AU - McHardy, Alice C.

AU - Schulze-Lefert, Paul

N1 - Copyright © 2015 The Authors. Published by Elsevier Inc. All rights reserved.

PY - 2015/3/11

Y1 - 2015/3/11

N2 - The microbial communities inhabiting the root interior of healthy plants, as well as the rhizosphere, which consists of soil particles firmly attached to roots, engage in symbiotic associations with their host. To investigate the structural and functional diversification among these communities, we employed a combination of 16S rRNA gene profiling and shotgun metagenome analysis of the microbiota associated with wild and domesticated accessions of barley (Hordeum vulgare). Bacterial families Comamonadaceae, Flavobacteriaceae, and Rhizobiaceae dominate the barley root-enriched microbiota. Host genotype has a small, but significant, effect on the diversity of root-associated bacterial communities, possibly representing a footprint of barley domestication. Traits related to pathogenesis, secretion, phage interactions, and nutrient mobilization are enriched in the barley root-associated microbiota. Strikingly, protein families assigned to these same traits showed evidence of positive selection. Our results indicate that the combined action of microbe-microbe and host-microbe interactions drives microbiota differentiation at the root-soil interface.

AB - The microbial communities inhabiting the root interior of healthy plants, as well as the rhizosphere, which consists of soil particles firmly attached to roots, engage in symbiotic associations with their host. To investigate the structural and functional diversification among these communities, we employed a combination of 16S rRNA gene profiling and shotgun metagenome analysis of the microbiota associated with wild and domesticated accessions of barley (Hordeum vulgare). Bacterial families Comamonadaceae, Flavobacteriaceae, and Rhizobiaceae dominate the barley root-enriched microbiota. Host genotype has a small, but significant, effect on the diversity of root-associated bacterial communities, possibly representing a footprint of barley domestication. Traits related to pathogenesis, secretion, phage interactions, and nutrient mobilization are enriched in the barley root-associated microbiota. Strikingly, protein families assigned to these same traits showed evidence of positive selection. Our results indicate that the combined action of microbe-microbe and host-microbe interactions drives microbiota differentiation at the root-soil interface.

UR - https://www.scopus.com/pages/publications/84926245612

U2 - 10.1016/j.chom.2015.01.011

DO - 10.1016/j.chom.2015.01.011

M3 - Article

C2 - 25732064

SN - 1931-3128

VL - 17

SP - 392

EP - 403

JO - Cell Host & Microbe

JF - Cell Host & Microbe

IS - 3

ER -