Structure and operation of the DNA-translocating type I DNA restriction enzymes

Christopher K. Kennaway, James E. Taylor, Chun Feng Song, Wojciech Potrzebowski, William Nicholson, John H. White, Anna Swiderska, Agnieszka Obarska-Kosinska, Philip Callow, Laurie P. Cooper, Gareth A. Roberts, Jean-Baptiste Artero, Janusz M. Bujnicki, John Trinick, G. Geoff Kneale, David T.F. Dryden (Lead / Corresponding author)

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Type I DNA restriction/modification (RM) enzymes are molecular machines found in the majority of bacterial species. Their early discovery paved the way for the development of genetic engineering. They control (restrict) the influx of foreign DNA via horizontal gene transfer into the bacterium while maintaining sequence-specific methylation (modification) of host DNA. The endonuclease reaction of these enzymes on unmethylated DNA is preceded by bidirectional translocation of thousands of base pairs of DNA toward the enzyme. We present the structures of two type I RM enzymes, EcoKI and EcoR124I, derived using electron microscopy (EM), small-angle scattering (neutron and X-ray), and detailed molecular modeling. DNA binding triggers a large contraction of the open form of the enzyme to a compact form. The path followed by DNA through the complexes is revealed by using a DNA mimic anti-restriction protein. The structures reveal an evolutionary link between type I RM enzymes and type II RM enzymes.
Original languageEnglish
Pages (from-to)92-104
Number of pages13
JournalGenes and Development
Volume26
DOIs
Publication statusPublished - 2012

Fingerprint

Type I Site Specific Deoxyribonucleases
DNA
Type II Site Specific Deoxyribonucleases
Enzymes
DNA Restriction-Modification Enzymes
Small Angle Scattering
Horizontal Gene Transfer
Genetic Engineering
Endonucleases
Neutrons
Base Pairing
Methylation
Electron Microscopy
X-Rays
Bacteria

Keywords

  • DNA restriction enzymes
  • DNA TRANSLOCATION
  • electron microscopy
  • small-angle scattering
  • Molecular modeling
  • DNA translocases

Cite this

Kennaway, Christopher K. ; Taylor, James E. ; Song, Chun Feng ; Potrzebowski, Wojciech ; Nicholson, William ; White, John H. ; Swiderska, Anna ; Obarska-Kosinska, Agnieszka ; Callow, Philip ; Cooper, Laurie P. ; Roberts, Gareth A. ; Artero, Jean-Baptiste ; Bujnicki, Janusz M. ; Trinick, John ; Kneale, G. Geoff ; Dryden, David T.F. / Structure and operation of the DNA-translocating type I DNA restriction enzymes. In: Genes and Development. 2012 ; Vol. 26. pp. 92-104.
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Kennaway, CK, Taylor, JE, Song, CF, Potrzebowski, W, Nicholson, W, White, JH, Swiderska, A, Obarska-Kosinska, A, Callow, P, Cooper, LP, Roberts, GA, Artero, J-B, Bujnicki, JM, Trinick, J, Kneale, GG & Dryden, DTF 2012, 'Structure and operation of the DNA-translocating type I DNA restriction enzymes', Genes and Development, vol. 26, pp. 92-104. https://doi.org/10.1101/gad.179085.111

Structure and operation of the DNA-translocating type I DNA restriction enzymes. / Kennaway, Christopher K.; Taylor, James E.; Song, Chun Feng; Potrzebowski, Wojciech; Nicholson, William; White, John H.; Swiderska, Anna; Obarska-Kosinska, Agnieszka; Callow, Philip; Cooper, Laurie P.; Roberts, Gareth A.; Artero, Jean-Baptiste; Bujnicki, Janusz M.; Trinick, John; Kneale, G. Geoff; Dryden, David T.F. (Lead / Corresponding author).

In: Genes and Development, Vol. 26, 2012, p. 92-104.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Structure and operation of the DNA-translocating type I DNA restriction enzymes

AU - Kennaway, Christopher K.

AU - Taylor, James E.

AU - Song, Chun Feng

AU - Potrzebowski, Wojciech

AU - Nicholson, William

AU - White, John H.

AU - Swiderska, Anna

AU - Obarska-Kosinska, Agnieszka

AU - Callow, Philip

AU - Cooper, Laurie P.

AU - Roberts, Gareth A.

AU - Artero, Jean-Baptiste

AU - Bujnicki, Janusz M.

AU - Trinick, John

AU - Kneale, G. Geoff

AU - Dryden, David T.F.

PY - 2012

Y1 - 2012

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AB - Type I DNA restriction/modification (RM) enzymes are molecular machines found in the majority of bacterial species. Their early discovery paved the way for the development of genetic engineering. They control (restrict) the influx of foreign DNA via horizontal gene transfer into the bacterium while maintaining sequence-specific methylation (modification) of host DNA. The endonuclease reaction of these enzymes on unmethylated DNA is preceded by bidirectional translocation of thousands of base pairs of DNA toward the enzyme. We present the structures of two type I RM enzymes, EcoKI and EcoR124I, derived using electron microscopy (EM), small-angle scattering (neutron and X-ray), and detailed molecular modeling. DNA binding triggers a large contraction of the open form of the enzyme to a compact form. The path followed by DNA through the complexes is revealed by using a DNA mimic anti-restriction protein. The structures reveal an evolutionary link between type I RM enzymes and type II RM enzymes.

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KW - electron microscopy

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KW - DNA translocases

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