Structure of a rare non-standard sequence k-turn bound by L7Ae protein

Lin Huang, David M J Lilley (Lead / Corresponding author)

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    8 Citations (Scopus)
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    Abstract

    Kt-23 from Thelohania solenopsae is a rare RNA kink turn (k-turn) where an adenine replaces the normal guanine at the 2n position. L7Ae is a member of a strongly conserved family of proteins that bind a range of k-turn structures in the ribosome, box C/D and H/ACA small nucleolar RNAs and U4 small nuclear RNA. We have solved the crystal structure of T. solenopsae Kt-23 RNA bound to Archeoglobus fulgidus L7Ae protein at a resolution of 2.95 Å. The protein binds in the major groove displayed on the outer face of the k-turn, in a manner similar to complexes with standard k-turn structures. The k-turn adopts a standard N3 class conformation, with a single hydrogen bond from A2b N6 to A2n N3. This contrasts with the structure of the same sequence located in the SAM-I riboswitch, where it adopts an N1 structure, showing the inherent plasticity of k-turn structure. This potentially can affect any tertiary interactions in which the RNA participates. © The Author(s) 2014. Published by Oxford University Press

    Original languageEnglish
    Pages (from-to)4734-4740
    Number of pages7
    JournalNucleic Acids Research
    Volume42
    Issue number7
    DOIs
    Publication statusPublished - Apr 2014

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