Projects per year
Abstract
ATP-dependent chromatin remodelling proteins represent a diverse family of proteins that share ATPase domains that are adapted to regulate protein-DNA interactions. Here we present structures of the Saccharomyces cerevisiae Chd1 protein engaged with nucleosomes in the presence of the transition state mimic ADP-beryllium fluoride. The path of DNA strands through the ATPase domains indicates the presence of contacts conserved with single strand translocases and additional contacts with both strands that are unique to Snf2 related proteins. The structure provides connectivity between rearrangement of ATPase lobes to a closed, nucleotide bound state and the sensing of linker DNA. Two turns of linker DNA are prised off the surface of the histone octamer as a result of Chd1 binding, and both the histone H3 tail and ubiquitin conjugated to lysine 120 are re-orientated towards the unravelled DNA. This indicates how changes to nucleosome structure can alter the way in which histone epitopes are presented.
Original language | English |
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Article number | e35720 |
Pages (from-to) | 1-28 |
Number of pages | 28 |
Journal | eLife |
Volume | 2018 |
Issue number | 7 |
Early online date | 6 Aug 2018 |
DOIs | |
Publication status | Published - 6 Aug 2018 |
ASJC Scopus subject areas
- General Neuroscience
- General Biochemistry,Genetics and Molecular Biology
- General Immunology and Microbiology
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Dive into the research topics of 'Structure of the chromatin remodelling enzyme Chd1 bound to a ubiquitinylated nucleosome'. Together they form a unique fingerprint.Projects
- 4 Finished
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Strategic Award: Wellcome Trust Technology Platform
Blow, J. (Investigator), Lamond, A. (Investigator) & Owen-Hughes, T. (Investigator)
1/01/13 → 30/09/18
Project: Research
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State of the Art Pulse EPR Instrumentation for Long Range Distance Measurements in Biomacromolecules (Multi-User Equipment Grant) (Joint with University of St Andrews)
Hunter, B. (Investigator), Keeble, D. (Investigator), Lilley, D. (Investigator), Norman, D. (Investigator) & Owen-Hughes, T. (Investigator)
24/09/12 → 23/09/17
Project: Research
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State-of-the-Art Facilities for Structural Biology at the University of Dundee
Hunter, B. (Investigator), Lilley, D. (Investigator), Owen-Hughes, T. (Investigator), Wyatt, P. (Investigator) & van Aalten, D. (Investigator)
1/03/12 → 28/02/17
Project: Research
Profiles
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Owen-Hughes, Tom
- Molecular Cell and Developmental Biology - Professor of Chromatin Structure and Dynamics
Person: Academic