TY - JOUR
T1 - Structure of the glycosylphosphatidylinositol membrane anchor of human placental alkaline phosphatase
AU - Redman, Christopher A.
AU - Thomas-Oates, Jane E.
AU - Ogata, Shigenori
AU - Ikehara, Yukio
AU - Ferguson, Michael A. J.
N1 - Medline is the source for the MeSH terms of this document.
PY - 1994/1/1
Y1 - 1994/1/1
N2 - The glycosylphosphatidylinositol membrane anchor of human placental alkaline phosphatase was isolated by exhaustive proteolysis followed by hydrophobic interaction chromatography. The resulting glycosylphosphatidylinositol-peptide was subjected to compositional analysis and chemical and enzymic modifications. The neutral-glycan fraction, prepared by dephosphorylation followed by HNO2 deamination and reduction, was sequenced using exoglycosidases and acetolysis. The phosphatidylinositol moiety was analysed by fast-atom bombardment mass spectrometry and gas chromatography-mass spectrometry. Taken together the data suggest the structure, Thr-Asp-ethanolamine-PO4-Man alpha 1-2Man alpha 1-6Man alpha 1-4GlcN-(sn-1-O- alkyl-2-O-acylglycerol-3-PO4-1-myo-D-inositol), which contains an additional ethanolamine phosphate group at an unknown position.
AB - The glycosylphosphatidylinositol membrane anchor of human placental alkaline phosphatase was isolated by exhaustive proteolysis followed by hydrophobic interaction chromatography. The resulting glycosylphosphatidylinositol-peptide was subjected to compositional analysis and chemical and enzymic modifications. The neutral-glycan fraction, prepared by dephosphorylation followed by HNO2 deamination and reduction, was sequenced using exoglycosidases and acetolysis. The phosphatidylinositol moiety was analysed by fast-atom bombardment mass spectrometry and gas chromatography-mass spectrometry. Taken together the data suggest the structure, Thr-Asp-ethanolamine-PO4-Man alpha 1-2Man alpha 1-6Man alpha 1-4GlcN-(sn-1-O- alkyl-2-O-acylglycerol-3-PO4-1-myo-D-inositol), which contains an additional ethanolamine phosphate group at an unknown position.
UR - http://www.scopus.com/inward/record.url?scp=0027939835&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0027939835
SN - 0264-6021
VL - 302
SP - 861
EP - 865
JO - Biochemical Journal
JF - Biochemical Journal
IS - 3
ER -