Structure of the glycosylphosphatidylinositol membrane anchor of human placental alkaline phosphatase

Christopher A. Redman; Jane E. Thomas-Oates; Shigenori Ogata; Yukio Ikehara; Michael A. J. Ferguson

Structure of the glycosylphosphatidylinositol membrane anchor of human placental alkaline phosphatase

Christopher A. Redman
, Jane E. Thomas-Oates
, Shigenori Ogata
, Yukio Ikehara
, Michael A. J. Ferguson

Research output: Contribution to journal › Article › peer-review

Abstract

The glycosylphosphatidylinositol membrane anchor of human placental alkaline phosphatase was isolated by exhaustive proteolysis followed by hydrophobic interaction chromatography. The resulting glycosylphosphatidylinositol-peptide was subjected to compositional analysis and chemical and enzymic modifications. The neutral-glycan fraction, prepared by dephosphorylation followed by HNO2 deamination and reduction, was sequenced using exoglycosidases and acetolysis. The phosphatidylinositol moiety was analysed by fast-atom bombardment mass spectrometry and gas chromatography-mass spectrometry. Taken together the data suggest the structure, Thr-Asp-ethanolamine-PO4-Man alpha 1-2Man alpha 1-6Man alpha 1-4GlcN-(sn-1-O- alkyl-2-O-acylglycerol-3-PO4-1-myo-D-inositol), which contains an additional ethanolamine phosphate group at an unknown position.

Original language	English
Pages (from-to)	861-865
Number of pages	5
Journal	Biochemical Journal
Volume	302
Issue number	3
Publication status	Published - 1 Jan 1994

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@article{c7462dbffae24ab2824ae77e2cc09448,

title = "Structure of the glycosylphosphatidylinositol membrane anchor of human placental alkaline phosphatase",

abstract = "The glycosylphosphatidylinositol membrane anchor of human placental alkaline phosphatase was isolated by exhaustive proteolysis followed by hydrophobic interaction chromatography. The resulting glycosylphosphatidylinositol-peptide was subjected to compositional analysis and chemical and enzymic modifications. The neutral-glycan fraction, prepared by dephosphorylation followed by HNO2 deamination and reduction, was sequenced using exoglycosidases and acetolysis. The phosphatidylinositol moiety was analysed by fast-atom bombardment mass spectrometry and gas chromatography-mass spectrometry. Taken together the data suggest the structure, Thr-Asp-ethanolamine-PO4-Man alpha 1-2Man alpha 1-6Man alpha 1-4GlcN-(sn-1-O- alkyl-2-O-acylglycerol-3-PO4-1-myo-D-inositol), which contains an additional ethanolamine phosphate group at an unknown position.",

author = "Redman, \{Christopher A.\} and Thomas-Oates, \{Jane E.\} and Shigenori Ogata and Yukio Ikehara and Ferguson, \{Michael A. J.\}",

note = "Medline is the source for the MeSH terms of this document.",

year = "1994",

month = jan,

day = "1",

language = "English",

volume = "302",

pages = "861--865",

journal = "Biochemical Journal",

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T1 - Structure of the glycosylphosphatidylinositol membrane anchor of human placental alkaline phosphatase

AU - Redman, Christopher A.

AU - Thomas-Oates, Jane E.

AU - Ogata, Shigenori

AU - Ikehara, Yukio

AU - Ferguson, Michael A. J.

N1 - Medline is the source for the MeSH terms of this document.

PY - 1994/1/1

Y1 - 1994/1/1

N2 - The glycosylphosphatidylinositol membrane anchor of human placental alkaline phosphatase was isolated by exhaustive proteolysis followed by hydrophobic interaction chromatography. The resulting glycosylphosphatidylinositol-peptide was subjected to compositional analysis and chemical and enzymic modifications. The neutral-glycan fraction, prepared by dephosphorylation followed by HNO2 deamination and reduction, was sequenced using exoglycosidases and acetolysis. The phosphatidylinositol moiety was analysed by fast-atom bombardment mass spectrometry and gas chromatography-mass spectrometry. Taken together the data suggest the structure, Thr-Asp-ethanolamine-PO4-Man alpha 1-2Man alpha 1-6Man alpha 1-4GlcN-(sn-1-O- alkyl-2-O-acylglycerol-3-PO4-1-myo-D-inositol), which contains an additional ethanolamine phosphate group at an unknown position.

AB - The glycosylphosphatidylinositol membrane anchor of human placental alkaline phosphatase was isolated by exhaustive proteolysis followed by hydrophobic interaction chromatography. The resulting glycosylphosphatidylinositol-peptide was subjected to compositional analysis and chemical and enzymic modifications. The neutral-glycan fraction, prepared by dephosphorylation followed by HNO2 deamination and reduction, was sequenced using exoglycosidases and acetolysis. The phosphatidylinositol moiety was analysed by fast-atom bombardment mass spectrometry and gas chromatography-mass spectrometry. Taken together the data suggest the structure, Thr-Asp-ethanolamine-PO4-Man alpha 1-2Man alpha 1-6Man alpha 1-4GlcN-(sn-1-O- alkyl-2-O-acylglycerol-3-PO4-1-myo-D-inositol), which contains an additional ethanolamine phosphate group at an unknown position.

UR - https://www.scopus.com/pages/publications/0027939835

M3 - Article

AN - SCOPUS:0027939835

SN - 0264-6021

VL - 302

SP - 861

EP - 865

JO - Biochemical Journal

JF - Biochemical Journal

IS - 3

ER -

Structure of the glycosylphosphatidylinositol membrane anchor of human placental alkaline phosphatase

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