Structure of the mouse calpain small subunit gene

J. Simon C. Arthur; Peter A. Greer; John S. Elce

doi:10.1016/S0167-4838(98)00166-6

Structure of the mouse calpain small subunit gene

J. Simon C. Arthur, Peter A. Greer, John S. Elce

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

The calpains comprise a family of heterodimeric (80+28 kDa) Ca²⁺-dependent cysteine proteases, probably having roles in signal transduction and cytoskeletal remodelling. We describe cloning and sequencing of the 28 kDa calpain subunit cDNA from mouse (coding for 268 amino acids), and characterization of its gene. The gene spans 7 kb and contains 11 exons. The promoter region, like those of other calpain genes, lacks an obvious TATA box, but contains several Sp1 binding sites.

Original language	English
Pages (from-to)	247-252
Number of pages	6
Journal	Biochimica et Biophysica Acta. Protein Structure and Molecular Enzymology
Volume	1388
Issue number	1
DOIs	https://doi.org/10.1016/S0167-4838(98)00166-6
Publication status	Published - 14 Oct 1998

Keywords

Polymerase Chain Reaction
DNA, Complementary
Animals
Promoter Regions, Genetic
Base Sequence
Blotting, Southern
Molecular Sequence Data
Calpain
Mice
Amino Acid Sequence

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10.1016/S0167-4838(98)00166-6

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AU - Arthur, J. Simon C.

AU - Greer, Peter A.

AU - Elce, John S.

PY - 1998/10/14

Y1 - 1998/10/14

N2 - The calpains comprise a family of heterodimeric (80+28 kDa) Ca2+-dependent cysteine proteases, probably having roles in signal transduction and cytoskeletal remodelling. We describe cloning and sequencing of the 28 kDa calpain subunit cDNA from mouse (coding for 268 amino acids), and characterization of its gene. The gene spans 7 kb and contains 11 exons. The promoter region, like those of other calpain genes, lacks an obvious TATA box, but contains several Sp1 binding sites.

AB - The calpains comprise a family of heterodimeric (80+28 kDa) Ca2+-dependent cysteine proteases, probably having roles in signal transduction and cytoskeletal remodelling. We describe cloning and sequencing of the 28 kDa calpain subunit cDNA from mouse (coding for 268 amino acids), and characterization of its gene. The gene spans 7 kb and contains 11 exons. The promoter region, like those of other calpain genes, lacks an obvious TATA box, but contains several Sp1 binding sites.

KW - Polymerase Chain Reaction

KW - DNA, Complementary

KW - Animals

KW - Promoter Regions, Genetic

KW - Base Sequence

KW - Blotting, Southern

KW - Molecular Sequence Data

KW - Calpain

KW - Mice

KW - Amino Acid Sequence

U2 - 10.1016/S0167-4838(98)00166-6

DO - 10.1016/S0167-4838(98)00166-6

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SN - 0167-4838

VL - 1388

SP - 247

EP - 252

JO - Biochimica et Biophysica Acta. Protein Structure and Molecular Enzymology

JF - Biochimica et Biophysica Acta. Protein Structure and Molecular Enzymology

IS - 1

ER -

Structure of the mouse calpain small subunit gene

Abstract

Keywords

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