The phosphosaccharide-inositol core of the lipophosphoglycan of Leishmania donovani was generated by treatment of the glycoconjugate with mild acid and digestion with phosphatidylinositol-specific phospholipase C. The core was purified and examined by one- and two-dimensional 1H-1H NMR and by methylation analysis. From the results, the carbohydrate core was elucidated as a phosphosaccharide attached to the inositol residue of the lyso-alkylphosphatidylinositol anchor of lipophosphoglycan as follows: PO4?6Galp(a1?6)Galp(a1?3)Galf(a1?3)Manp(a1?3)Manp(a1?4)GlcNp(a1?6)myo-inositol. The presence of an internal galactofuranose residue is highly unusual and the Manp(a1?4)GlcNp(a1?6)myo-inositol sequence is homologous to the respective portion of the glycosylphosphatidylinositol anchors reported for both the Trypanosoma brucei variant surface glycoprotein and the rat brain Thy-1 glycoprotein.
|Number of pages||5|
|Journal||Journal of Biological Chemistry|
|Publication status||Published - 25 Apr 1989|