Structure of the phosphosaccharide-inositol core of the Leishmania donovani lipophosphoglycan

Salvatore J. Turco; Palmer A. Orlandi Jr.; Steven W. Homans; Michael A. J. Ferguson; Raymond A. Dwek; Thomas W. Rademacher

Structure of the phosphosaccharide-inositol core of the Leishmania donovani lipophosphoglycan

Salvatore J. Turco, Palmer A. Orlandi Jr., Steven W. Homans, Michael A. J. Ferguson, Raymond A. Dwek, Thomas W. Rademacher

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Abstract

The phosphosaccharide-inositol core of the lipophosphoglycan of Leishmania donovani was generated by treatment of the glycoconjugate with mild acid and digestion with phosphatidylinositol-specific phospholipase C. The core was purified and examined by one- and two-dimensional ¹H-¹H NMR and by methylation analysis. From the results, the carbohydrate core was elucidated as a phosphosaccharide attached to the inositol residue of the lyso-alkylphosphatidylinositol anchor of lipophosphoglycan as follows: PO₄?6Gal_p(a1?6)Gal_p(a1?3)Gal_f(a1?3)Man_p(a1?3)Man_p(a1?4)GlcN_p(a1?6)myo-inositol. The presence of an internal galactofuranose residue is highly unusual and the Man_p(a1?4)GlcN_p(a1?6)myo-inositol sequence is homologous to the respective portion of the glycosylphosphatidylinositol anchors reported for both the Trypanosoma brucei variant surface glycoprotein and the rat brain Thy-1 glycoprotein.

Original language	English
Pages (from-to)	6711-6715
Number of pages	5
Journal	Journal of Biological Chemistry
Volume	264
Issue number	12
Publication status	Published - 25 Apr 1989

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title = "Structure of the phosphosaccharide-inositol core of the Leishmania donovani lipophosphoglycan",

abstract = "The phosphosaccharide-inositol core of the lipophosphoglycan of Leishmania donovani was generated by treatment of the glycoconjugate with mild acid and digestion with phosphatidylinositol-specific phospholipase C. The core was purified and examined by one- and two-dimensional 1H-1H NMR and by methylation analysis. From the results, the carbohydrate core was elucidated as a phosphosaccharide attached to the inositol residue of the lyso-alkylphosphatidylinositol anchor of lipophosphoglycan as follows: PO4?6Galp(a1?6)Galp(a1?3)Galf(a1?3)Manp(a1?3)Manp(a1?4)GlcNp(a1?6)myo-inositol. The presence of an internal galactofuranose residue is highly unusual and the Manp(a1?4)GlcNp(a1?6)myo-inositol sequence is homologous to the respective portion of the glycosylphosphatidylinositol anchors reported for both the Trypanosoma brucei variant surface glycoprotein and the rat brain Thy-1 glycoprotein.",

author = "Turco, {Salvatore J.} and {Orlandi Jr.}, {Palmer A.} and Homans, {Steven W.} and Ferguson, {Michael A. J.} and Dwek, {Raymond A.} and Rademacher, {Thomas W.}",

year = "1989",

month = apr,

day = "25",

language = "English",

volume = "264",

pages = "6711--6715",

journal = "Journal of Biological Chemistry",

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TY - JOUR

T1 - Structure of the phosphosaccharide-inositol core of the Leishmania donovani lipophosphoglycan

AU - Turco, Salvatore J.

AU - Orlandi Jr., Palmer A.

AU - Homans, Steven W.

AU - Ferguson, Michael A. J.

AU - Dwek, Raymond A.

AU - Rademacher, Thomas W.

PY - 1989/4/25

Y1 - 1989/4/25

N2 - The phosphosaccharide-inositol core of the lipophosphoglycan of Leishmania donovani was generated by treatment of the glycoconjugate with mild acid and digestion with phosphatidylinositol-specific phospholipase C. The core was purified and examined by one- and two-dimensional 1H-1H NMR and by methylation analysis. From the results, the carbohydrate core was elucidated as a phosphosaccharide attached to the inositol residue of the lyso-alkylphosphatidylinositol anchor of lipophosphoglycan as follows: PO4?6Galp(a1?6)Galp(a1?3)Galf(a1?3)Manp(a1?3)Manp(a1?4)GlcNp(a1?6)myo-inositol. The presence of an internal galactofuranose residue is highly unusual and the Manp(a1?4)GlcNp(a1?6)myo-inositol sequence is homologous to the respective portion of the glycosylphosphatidylinositol anchors reported for both the Trypanosoma brucei variant surface glycoprotein and the rat brain Thy-1 glycoprotein.

AB - The phosphosaccharide-inositol core of the lipophosphoglycan of Leishmania donovani was generated by treatment of the glycoconjugate with mild acid and digestion with phosphatidylinositol-specific phospholipase C. The core was purified and examined by one- and two-dimensional 1H-1H NMR and by methylation analysis. From the results, the carbohydrate core was elucidated as a phosphosaccharide attached to the inositol residue of the lyso-alkylphosphatidylinositol anchor of lipophosphoglycan as follows: PO4?6Galp(a1?6)Galp(a1?3)Galf(a1?3)Manp(a1?3)Manp(a1?4)GlcNp(a1?6)myo-inositol. The presence of an internal galactofuranose residue is highly unusual and the Manp(a1?4)GlcNp(a1?6)myo-inositol sequence is homologous to the respective portion of the glycosylphosphatidylinositol anchors reported for both the Trypanosoma brucei variant surface glycoprotein and the rat brain Thy-1 glycoprotein.

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AN - SCOPUS:0024602370

SN - 0021-9258

VL - 264

SP - 6711

EP - 6715

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

IS - 12

ER -

Structure of the phosphosaccharide-inositol core of the Leishmania donovani lipophosphoglycan

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