Structure of the phosphosaccharide-inositol core of the Leishmania donovani lipophosphoglycan

Salvatore J. Turco, Palmer A. Orlandi Jr., Steven W. Homans, Michael A. J. Ferguson, Raymond A. Dwek, Thomas W. Rademacher

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    Abstract

    The phosphosaccharide-inositol core of the lipophosphoglycan of Leishmania donovani was generated by treatment of the glycoconjugate with mild acid and digestion with phosphatidylinositol-specific phospholipase C. The core was purified and examined by one- and two-dimensional 1H-1H NMR and by methylation analysis. From the results, the carbohydrate core was elucidated as a phosphosaccharide attached to the inositol residue of the lyso-alkylphosphatidylinositol anchor of lipophosphoglycan as follows: PO4?6Galp(a1?6)Galp(a1?3)Galf(a1?3)Manp(a1?3)Manp(a1?4)GlcNp(a1?6)myo-inositol. The presence of an internal galactofuranose residue is highly unusual and the Manp(a1?4)GlcNp(a1?6)myo-inositol sequence is homologous to the respective portion of the glycosylphosphatidylinositol anchors reported for both the Trypanosoma brucei variant surface glycoprotein and the rat brain Thy-1 glycoprotein.

    Original languageEnglish
    Pages (from-to)6711-6715
    Number of pages5
    JournalJournal of Biological Chemistry
    Volume264
    Issue number12
    Publication statusPublished - 25 Apr 1989

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