Studies on the structure of a phosphoglycoprotein from the parasitic protozoan Trypanosoma cruzi.

Michael A. J. Ferguson, Anthony K. Allen, David Snary

    Research output: Contribution to journalArticle

    40 Citations (Scopus)

    Abstract

    A glycoprotein (GP72) has been isolated from Trypanosoma cruzi and found to contain 41% protein, 49% carbohydrate and 10% phosphate. All phosphate was covalently attached to the carbohydrate which contained the following sugars: ribose, xylose, fucose, galactose, mannose, glucose and glucosamine. The carbohydrate side chains were linked to protein by fucose, xylose and N-acetylglucosamine; 50% of the total N-acetylglucosamine was involved in glycoprotein linkages. Two classes of carbohydrate side chains were detected. One class comprised 15% of the total carbohydrate and contained glucosamine, mannose and galactose; some of these chains were phosphorylated. The other class comprised 85% of the total carbohydrate and contained xylose, ribose, fucose, galactose, mannose, glucosamine and phosphate; these chains were antigenic and reacted with a monoclonal antibody with specificity for the whole glycoprotein.

    Original languageEnglish
    Pages (from-to)313-319
    Number of pages7
    JournalBiochemical Journal
    Volume213
    Issue number2
    Publication statusPublished - 1 Aug 1983

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