Studies on the structure of a phosphoglycoprotein from the parasitic protozoan Trypanosoma cruzi.

Michael A. J. Ferguson; Anthony K. Allen; David Snary

Studies on the structure of a phosphoglycoprotein from the parasitic protozoan Trypanosoma cruzi.

Michael A. J. Ferguson
, Anthony K. Allen
, David Snary

Research output: Contribution to journal › Article › peer-review

Abstract

A glycoprotein (GP72) has been isolated from Trypanosoma cruzi and found to contain 41% protein, 49% carbohydrate and 10% phosphate. All phosphate was covalently attached to the carbohydrate which contained the following sugars: ribose, xylose, fucose, galactose, mannose, glucose and glucosamine. The carbohydrate side chains were linked to protein by fucose, xylose and N-acetylglucosamine; 50% of the total N-acetylglucosamine was involved in glycoprotein linkages. Two classes of carbohydrate side chains were detected. One class comprised 15% of the total carbohydrate and contained glucosamine, mannose and galactose; some of these chains were phosphorylated. The other class comprised 85% of the total carbohydrate and contained xylose, ribose, fucose, galactose, mannose, glucosamine and phosphate; these chains were antigenic and reacted with a monoclonal antibody with specificity for the whole glycoprotein.

Original language	English
Pages (from-to)	313-319
Number of pages	7
Journal	Biochemical Journal
Volume	213
Issue number	2
Publication status	Published - 1 Aug 1983

Access to Document

http://www.biochemj.org/bj/213/bj2130313.htm

Cite this

@article{27eb0adb0a254e7ebda5d0f707f302da,

title = "Studies on the structure of a phosphoglycoprotein from the parasitic protozoan Trypanosoma cruzi.",

abstract = "A glycoprotein (GP72) has been isolated from Trypanosoma cruzi and found to contain 41\% protein, 49\% carbohydrate and 10\% phosphate. All phosphate was covalently attached to the carbohydrate which contained the following sugars: ribose, xylose, fucose, galactose, mannose, glucose and glucosamine. The carbohydrate side chains were linked to protein by fucose, xylose and N-acetylglucosamine; 50\% of the total N-acetylglucosamine was involved in glycoprotein linkages. Two classes of carbohydrate side chains were detected. One class comprised 15\% of the total carbohydrate and contained glucosamine, mannose and galactose; some of these chains were phosphorylated. The other class comprised 85\% of the total carbohydrate and contained xylose, ribose, fucose, galactose, mannose, glucosamine and phosphate; these chains were antigenic and reacted with a monoclonal antibody with specificity for the whole glycoprotein.",

author = "Ferguson, \{Michael A. J.\} and Allen, \{Anthony K.\} and David Snary",

year = "1983",

month = aug,

day = "1",

language = "English",

volume = "213",

pages = "313--319",

journal = "Biochemical Journal",

issn = "0264-6021",

publisher = "Portland Press",

number = "2",

}

TY - JOUR

T1 - Studies on the structure of a phosphoglycoprotein from the parasitic protozoan Trypanosoma cruzi.

AU - Ferguson, Michael A. J.

AU - Allen, Anthony K.

AU - Snary, David

PY - 1983/8/1

Y1 - 1983/8/1

N2 - A glycoprotein (GP72) has been isolated from Trypanosoma cruzi and found to contain 41% protein, 49% carbohydrate and 10% phosphate. All phosphate was covalently attached to the carbohydrate which contained the following sugars: ribose, xylose, fucose, galactose, mannose, glucose and glucosamine. The carbohydrate side chains were linked to protein by fucose, xylose and N-acetylglucosamine; 50% of the total N-acetylglucosamine was involved in glycoprotein linkages. Two classes of carbohydrate side chains were detected. One class comprised 15% of the total carbohydrate and contained glucosamine, mannose and galactose; some of these chains were phosphorylated. The other class comprised 85% of the total carbohydrate and contained xylose, ribose, fucose, galactose, mannose, glucosamine and phosphate; these chains were antigenic and reacted with a monoclonal antibody with specificity for the whole glycoprotein.

AB - A glycoprotein (GP72) has been isolated from Trypanosoma cruzi and found to contain 41% protein, 49% carbohydrate and 10% phosphate. All phosphate was covalently attached to the carbohydrate which contained the following sugars: ribose, xylose, fucose, galactose, mannose, glucose and glucosamine. The carbohydrate side chains were linked to protein by fucose, xylose and N-acetylglucosamine; 50% of the total N-acetylglucosamine was involved in glycoprotein linkages. Two classes of carbohydrate side chains were detected. One class comprised 15% of the total carbohydrate and contained glucosamine, mannose and galactose; some of these chains were phosphorylated. The other class comprised 85% of the total carbohydrate and contained xylose, ribose, fucose, galactose, mannose, glucosamine and phosphate; these chains were antigenic and reacted with a monoclonal antibody with specificity for the whole glycoprotein.

UR - https://www.scopus.com/pages/publications/0020807337

M3 - Article

SN - 0264-6021

VL - 213

SP - 313

EP - 319

JO - Biochemical Journal

JF - Biochemical Journal

IS - 2

ER -

Studies on the structure of a phosphoglycoprotein from the parasitic protozoan Trypanosoma cruzi.

Abstract

Access to Document

Other files and links

Fingerprint

Cite this