Studying protein-DNA binding by dynamic light scattering

N. Zh Zhelev, R. S. Buckle, D. Snyder, P. J. Marsh

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Light scattering experiments were undertaken to study binding of the Max transcription factor to its E-box DNA recognition sequence. Translational diffusion coefficients were measured and the average hydrodynamic radii (Rh) of complexes calculated using the Stokes-Einstein equation. We detected both dimerization of Max and the formation of a stable complex with its E-box DNA target. These results demonstrate the applicability of Dynamic Light Scattering for measuring protein-DNA interactions.

Original languageEnglish
Pages (from-to)199-203
Number of pages5
JournalCellular and Molecular Biology Letters
Issue number2
Publication statusPublished - Jun 1996


  • Dynamic light scattering
  • Max
  • Protein-DNA interactions

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology


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