Light scattering experiments were undertaken to study binding of the Max transcription factor to its E-box DNA recognition sequence. Translational diffusion coefficients were measured and the average hydrodynamic radii (Rh) of complexes calculated using the Stokes-Einstein equation. We detected both dimerization of Max and the formation of a stable complex with its E-box DNA target. These results demonstrate the applicability of Dynamic Light Scattering for measuring protein-DNA interactions.
|Number of pages||5|
|Journal||Cellular and Molecular Biology Letters|
|Publication status||Published - Jun 1996|
- Dynamic light scattering
- Protein-DNA interactions