Studying protein-DNA binding by dynamic light scattering

N. Zh Zhelev; R. S. Buckle; D. Snyder; P. J. Marsh

Studying protein-DNA binding by dynamic light scattering

N. Zh Zhelev, R. S. Buckle, D. Snyder, P. J. Marsh

Research output: Contribution to journal › Article › peer-review

Abstract

Light scattering experiments were undertaken to study binding of the Max transcription factor to its E-box DNA recognition sequence. Translational diffusion coefficients were measured and the average hydrodynamic radii (R_h) of complexes calculated using the Stokes-Einstein equation. We detected both dimerization of Max and the formation of a stable complex with its E-box DNA target. These results demonstrate the applicability of Dynamic Light Scattering for measuring protein-DNA interactions.

Original language	English
Pages (from-to)	199-203
Number of pages	5
Journal	Cellular and Molecular Biology Letters
Volume	1
Issue number	2
Publication status	Published - Jun 1996

Keywords

Dynamic light scattering
Max
Protein-DNA interactions

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Cell Biology

Cite this

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title = "Studying protein-DNA binding by dynamic light scattering",

abstract = "Light scattering experiments were undertaken to study binding of the Max transcription factor to its E-box DNA recognition sequence. Translational diffusion coefficients were measured and the average hydrodynamic radii (Rh) of complexes calculated using the Stokes-Einstein equation. We detected both dimerization of Max and the formation of a stable complex with its E-box DNA target. These results demonstrate the applicability of Dynamic Light Scattering for measuring protein-DNA interactions.",

keywords = "Dynamic light scattering, Max, Protein-DNA interactions",

author = "Zhelev, {N. Zh} and Buckle, {R. S.} and D. Snyder and Marsh, {P. J.}",

year = "1996",

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journal = "Cellular and Molecular Biology Letters",

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AU - Zhelev, N. Zh

AU - Buckle, R. S.

AU - Snyder, D.

AU - Marsh, P. J.

PY - 1996/6

Y1 - 1996/6

N2 - Light scattering experiments were undertaken to study binding of the Max transcription factor to its E-box DNA recognition sequence. Translational diffusion coefficients were measured and the average hydrodynamic radii (Rh) of complexes calculated using the Stokes-Einstein equation. We detected both dimerization of Max and the formation of a stable complex with its E-box DNA target. These results demonstrate the applicability of Dynamic Light Scattering for measuring protein-DNA interactions.

AB - Light scattering experiments were undertaken to study binding of the Max transcription factor to its E-box DNA recognition sequence. Translational diffusion coefficients were measured and the average hydrodynamic radii (Rh) of complexes calculated using the Stokes-Einstein equation. We detected both dimerization of Max and the formation of a stable complex with its E-box DNA target. These results demonstrate the applicability of Dynamic Light Scattering for measuring protein-DNA interactions.

KW - Dynamic light scattering

KW - Max

KW - Protein-DNA interactions

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UR - http://www.cmbl.org.pl/vol1_nr2.php

M3 - Article

AN - SCOPUS:3142632242

SN - 1425-8153

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JO - Cellular and Molecular Biology Letters

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ER -

Studying protein-DNA binding by dynamic light scattering

Abstract

Keywords

ASJC Scopus subject areas

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