Subcellular localization and trafficking of phytolongins (non-SNARE longins) in the plant secretory pathway

Carine De Marcos Lousa, Eric Soubeyrand, Paolo Bolognese, Valerie Wattelet-Boyer, Guillaume Bouyssou, Claireline Marais, Yohann Boutte, Francesco Filippini, Patrick Moreau (Lead / Corresponding author)

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)
37 Downloads (Pure)

Abstract

SNARE proteins are central elements of the machinery involved in membrane fusion of eukaryotic cells. In animals and plants, SNAREs have diversified to sustain a variety of specific functions. In animals, R-SNARE proteins called brevins have diversified; in contrast, in plants, the R-SNARE proteins named longins have diversified. Recently, a new subfamily of four longins named 'phytolongins' (Phyl) was discovered. One intriguing aspect of Phyl proteins is the lack of the typical SNARE motif, which is replaced by another domain termed the 'Phyl domain'. Phytolongins have a rather ubiquitous tissue expression in Arabidopsis but still await intracellular characterization. In this study, we found that the four phytolongins are distributed along the secretory pathway. While Phyl2.1 and Phyl2.2 are strictly located at the endoplasmic reticulum network, Phyl1.2 associates with the Golgi bodies, and Phyl1.1 locates mainly at the plasma membrane and partially in the Golgi bodies and post-Golgi compartments. Our results show that export of Phyl1.1 from the endoplasmic reticulum depends on the GTPase Sar1, the Sar1 guanine nucleotide exchange factor Sec12, and the SNAREs Sec22 and Memb11. In addition, we have identified the Y48F49 motif as being critical for the exit of Phyl1.1 from the endoplasmic reticulum. Our results provide the first characterization of the subcellular localization of the phytolongins, and we discuss their potential role in regulating the secretory pathway.

Original languageEnglish
Pages (from-to)2627-2639
Number of pages13
JournalJournal of Experimental Botany
Volume67
Issue number9
Early online date9 Mar 2016
DOIs
Publication statusPublished - Apr 2016

Keywords

  • ER export YF motif
  • longin domain
  • phytolongins
  • protein targeting
  • secretory pathway
  • subcellular localization

ASJC Scopus subject areas

  • Physiology
  • Plant Science

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