Subcellular spatial resolution achieved for deep-brain imaging in vivo using a minimally invasive multimode fiber

Sebastian A. Vasquez-Lopez, Raphaël Turcotte, Vadim Koren, Martin Plöschner, Zahid Padamsey, Martin J. Booth, Tomáš Čižmár, Nigel J. Emptage (Lead / Corresponding author)

Research output: Contribution to journalLetterpeer-review

39 Citations (Scopus)
108 Downloads (Pure)

Abstract

Achieving intravital optical imaging with diffraction-limited spatial resolution of deep-brain structures represents an important step toward the goal of understanding the mammalian central nervous system1–4. Advances in wavefront-shaping methods and computational power have recently allowed for a novel approach to high-resolution imaging, utilizing deterministic light propagation through optically complex media and, of particular importance for this work, multimode optical fibers (MMFs)5–7. We report a compact and highly optimized approach for minimally invasive in vivo brain imaging applications. The volume of tissue lesion was reduced by more than 100-fold, while preserving diffraction-limited imaging performance utilizing wavefront control of light propagation through a single 50-μm-core MMF. Here, we demonstrated high-resolution fluorescence imaging of subcellular neuronal structures, dendrites and synaptic specializations, in deep-brain regions of living mice, as well as monitored stimulus-driven functional Ca2+ responses. These results represent a major breakthrough in the compromise between high-resolution imaging and tissue damage, heralding new possibilities for deep-brain imaging in vivo.

Original languageEnglish
Article number110
Pages (from-to)1-6
Number of pages6
JournalLight: Science and Applications
Volume7
Issue number1
Early online date19 Dec 2018
DOIs
Publication statusPublished - 19 Dec 2018

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