Subcellular spatial resolution achieved for deep-brain imaging in vivo using a minimally invasive multimode fiber

TY - JOUR

T1 - Subcellular spatial resolution achieved for deep-brain imaging in vivo using a minimally invasive multimode fiber

AU - Vasquez-Lopez, Sebastian A.

AU - Turcotte, Raphaël

AU - Koren, Vadim

AU - Plöschner, Martin

AU - Padamsey, Zahid

AU - Booth, Martin J.

AU - Čižmár, Tomáš

AU - Emptage, Nigel J.

N1 - M.P. and T.C. acknowledge support from the University of Dundee and Scottish Universities Physics Alliance (PaLS initiative). T.C. acknowledges support from the European Regional Development Fund, Project No. CZ.02.1.01/0.0/0.0/15 003/0000476. S.A.V.L., V.K., Z.P., R.T., M.J.B. and N.E. acknowledge support from the John Fell Fund, the BBSRC (TDRF) and the MRC (UK).

PY - 2018/12/19

Y1 - 2018/12/19

N2 - Achieving intravital optical imaging with diffraction-limited spatial resolution of deep-brain structures represents an important step toward the goal of understanding the mammalian central nervous system1–4. Advances in wavefront-shaping methods and computational power have recently allowed for a novel approach to high-resolution imaging, utilizing deterministic light propagation through optically complex media and, of particular importance for this work, multimode optical fibers (MMFs)5–7. We report a compact and highly optimized approach for minimally invasive in vivo brain imaging applications. The volume of tissue lesion was reduced by more than 100-fold, while preserving diffraction-limited imaging performance utilizing wavefront control of light propagation through a single 50-μm-core MMF. Here, we demonstrated high-resolution fluorescence imaging of subcellular neuronal structures, dendrites and synaptic specializations, in deep-brain regions of living mice, as well as monitored stimulus-driven functional Ca2+ responses. These results represent a major breakthrough in the compromise between high-resolution imaging and tissue damage, heralding new possibilities for deep-brain imaging in vivo.

AB - Achieving intravital optical imaging with diffraction-limited spatial resolution of deep-brain structures represents an important step toward the goal of understanding the mammalian central nervous system1–4. Advances in wavefront-shaping methods and computational power have recently allowed for a novel approach to high-resolution imaging, utilizing deterministic light propagation through optically complex media and, of particular importance for this work, multimode optical fibers (MMFs)5–7. We report a compact and highly optimized approach for minimally invasive in vivo brain imaging applications. The volume of tissue lesion was reduced by more than 100-fold, while preserving diffraction-limited imaging performance utilizing wavefront control of light propagation through a single 50-μm-core MMF. Here, we demonstrated high-resolution fluorescence imaging of subcellular neuronal structures, dendrites and synaptic specializations, in deep-brain regions of living mice, as well as monitored stimulus-driven functional Ca2+ responses. These results represent a major breakthrough in the compromise between high-resolution imaging and tissue damage, heralding new possibilities for deep-brain imaging in vivo.

UR - https://www.scopus.com/pages/publications/85058859483

U2 - 10.1038/s41377-018-0111-0

DO - 10.1038/s41377-018-0111-0

M3 - Letter

C2 - 30588295

AN - SCOPUS:85058859483

SN - 2095-5545

VL - 7

SP - 1

EP - 6

JO - Light: Science and Applications

JF - Light: Science and Applications

IS - 1

M1 - 110

ER -