Sugar mimics: an artificial receptor for cholera toxin

Anna Bernardi, Anna Checchia, Paola Brocca, Sandro Sonnino, Fabio Zuccotto

Research output: Contribution to journalArticlepeer-review

52 Citations (Scopus)

Abstract

The paper describes the pseudosugar 2 [Galβ1-3GalNAcβ1-4(NeuAcα2- 3)DCCHD], a high affinity binder of cholera toxin (CT). The molecule was designed using molecular modeling techniques to mimic the natural CT membrane receptor, ganglioside GM1. The central residue of GM1, a 3,4-disubstituted galactose unit, was recognized as the ganglioside scaffold element and substituted with a conformationally locked cyclohexanediol (DCCHD). DCCHD was synthesized in enantiopure form using enantioselective Diels Alder methodology and regioselectively α-sialylation at the equatorial position. Glycosylation with a Galβ(1-3)-GalNAc donor completed the synthesis of 2. The solution structure of 2 and its binding ability to CT were found to be analogous to those of the GM1 oligosaccharide.

Original languageEnglish
Pages (from-to)2032-2036
Number of pages5
JournalJournal of the American Chemical Society
Volume121
Issue number10
Early online date2 Mar 1999
DOIs
Publication statusPublished - 17 Mar 1999

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