Projects per year
The structural characterisation of protein-protein interactions is often challenging. Where interactions are not amenable to high-resolution approaches, alternatives providing lower resolution information are often of value. One such approach is site-directed cross-linking. Here, through the introduction of cysteine residues at strategic locations in histone proteins, we use site-directed cross-linking to monitor the association of chromatin subunits. This approach is informative for the study of both recombinant and native chromatin complexes consisting either of histone subunits alone or in association with accessory proteins, in this case histone chaperones. The approaches described may be generally applicable for monitoring the interactions of a diverse range of multi-protein complexes.
|Title of host publication||Chromatin remodeling|
|Subtitle of host publication||methods and protocols|
|Editors||Randall H. Morse|
|Number of pages||15|
|Publication status||Published - 2012|
|Name||Methods in Molecular Biology|
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- 1 Finished
1/01/12 → 31/05/19