Sumoylation regulates protein dynamics during meiotic chromosome segregation in C. elegans oocytes

Federico Pelisch (Lead / Corresponding author), Laura Bel Borja, Ellis Jaffray, Ronald Hay

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    17 Citations (Scopus)
    138 Downloads (Pure)


    Oocyte meiotic spindles in most species lack centrosomes and the mechanisms that underlie faithful chromosome segregation in acentrosomal meiotic spindles are not well understood. In C. elegans oocytes, spindle microtubules exert a poleward force on chromosomes that is dependent on the microtubule-stabilising protein CLS-2, the orthologue of the mammalian CLASP proteins. The checkpoint kinase BUB-1 and CLS-2 localise in the central spindle and display a dynamic localisation pattern throughout anaphase, but the signals regulating their anaphase-specific localisation remains unknown. We have shown previously that SUMO regulates BUB-1 localisation during metaphase I. Here, we found that SUMO modification of BUB-1 is regulated by the SUMO E3 ligase GEI-17 and the SUMO protease ULP-1. SUMO and GEI-17 are required for BUB-1 localisation between segregating chromosomes during early anaphase I. We also show that CLS-2 is subject to SUMO-mediated regulation; CLS-2 precociously localises in the midbivalent when either SUMO or GEI-17 are depleted. Overall, we provide evidence for a novel, SUMO-mediated control of protein dynamics during early anaphase I in oocytes.

    Original languageEnglish
    Article numberjcs232330
    Number of pages13
    JournalJournal of Cell Science
    Issue number14
    Early online date26 Jun 2019
    Publication statusPublished - 18 Jul 2019


    • meiosis
    • oocytes
    • SUMO
    • chromosomes
    • segregation
    • Spindle
    • Segregation
    • Meiosis
    • Chromosomes
    • Oocytes

    ASJC Scopus subject areas

    • Cell Biology


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