Projects per year
Abstract
Oocyte meiotic spindles in most species lack centrosomes and the mechanisms that underlie faithful chromosome segregation in acentrosomal meiotic spindles are not well understood. In C. elegans oocytes, spindle microtubules exert a poleward force on chromosomes that is dependent on the microtubule-stabilising protein CLS-2, the orthologue of the mammalian CLASP proteins. The checkpoint kinase BUB-1 and CLS-2 localise in the central spindle and display a dynamic localisation pattern throughout anaphase, but the signals regulating their anaphase-specific localisation remains unknown. We have shown previously that SUMO regulates BUB-1 localisation during metaphase I. Here, we found that SUMO modification of BUB-1 is regulated by the SUMO E3 ligase GEI-17 and the SUMO protease ULP-1. SUMO and GEI-17 are required for BUB-1 localisation between segregating chromosomes during early anaphase I. We also show that CLS-2 is subject to SUMO-mediated regulation; CLS-2 precociously localises in the midbivalent when either SUMO or GEI-17 are depleted. Overall, we provide evidence for a novel, SUMO-mediated control of protein dynamics during early anaphase I in oocytes.
Original language | English |
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Article number | jcs232330 |
Number of pages | 13 |
Journal | Journal of Cell Science |
Volume | 132 |
Issue number | 14 |
Early online date | 26 Jun 2019 |
DOIs | |
Publication status | Published - 18 Jul 2019 |
Keywords
- meiosis
- oocytes
- SUMO
- chromosomes
- segregation
- Spindle
- Segregation
- Meiosis
- Chromosomes
- Oocytes
ASJC Scopus subject areas
- Cell Biology
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Dive into the research topics of 'Sumoylation regulates protein dynamics during meiotic chromosome segregation in C. elegans oocytes'. Together they form a unique fingerprint.Projects
- 3 Finished
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Regulation of Meiotic Chromosome Segregation by Post-Translational Modifications
Pelisch, F. (Investigator)
14/05/18 → 13/11/23
Project: Research
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Harnessing the PML-SUMO-RNF4 Axis for Cancer Therapy (Programme Grant)
Hay, R. (Investigator)
1/12/16 → 30/09/22
Project: Research
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Determining the Role and Mechanism of Action of SUMO Targeted Ubiquitin Ligase RNF4 in Maintaining Genome Integrity (Senior Investigator Award)
Hay, R. (Investigator)
1/10/12 → 31/01/20
Project: Research
Profiles
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Pelisch, Fede
- Molecular Cell and Developmental Biology - Principal Investigator/Senior Lecturer (Teaching and Research)
Person: Academic