Super-resolution video microscopy of live cells by structured illumination

Peter Kner, Bryant B. Chhun, Eric R. Griffis, Lukman Winoto, Mats G. L. Gustafsson (Lead / Corresponding author)

    Research output: Contribution to journalArticle

    460 Citations (Scopus)

    Abstract

    Structured-illumination microscopy can double the resolution of the widefield fluorescence microscope but has previously been too slow for dynamic live imaging. Here we demonstrate a high-speed structured-illumination microscope that is capable of 100-nm resolution at frame rates up to 11 Hz for several hundred time points. We demonstrate the microscope by video imaging of tubulin and kinesin dynamics in living Drosophila melanogaster S2 cells in the total internal reflection mode.

    Original languageEnglish
    Pages (from-to)339-342
    Number of pages4
    JournalNature Methods
    Volume6
    Issue number5
    DOIs
    Publication statusPublished - May 2009

    Keywords

    • REFLECTION FLUORESCENCE MICROSCOPY
    • LOCALIZATION MICROSCOPY
    • SPINDLE
    • RESOLUTION
    • DYNAMICS
    • PROTEIN
    • KINETOCHORE
    • MITOSIS
    • LIMIT
    • FLUX

    Cite this

    Kner, P., Chhun, B. B., Griffis, E. R., Winoto, L., & Gustafsson, M. G. L. (2009). Super-resolution video microscopy of live cells by structured illumination. Nature Methods, 6(5), 339-342. https://doi.org/10.1038/NMETH.1324