Super-resolution video microscopy of live cells by structured illumination

Peter Kner; Bryant B. Chhun; Eric R. Griffis; Lukman Winoto; Mats G. L. Gustafsson

doi:10.1038/NMETH.1324

Super-resolution video microscopy of live cells by structured illumination

Peter Kner, Bryant B. Chhun, Eric R. Griffis, Lukman Winoto, Mats G. L. Gustafsson (Lead / Corresponding author)

Research output: Contribution to journal › Article › peer-review

641 Citations (Scopus)

Abstract

Structured-illumination microscopy can double the resolution of the widefield fluorescence microscope but has previously been too slow for dynamic live imaging. Here we demonstrate a high-speed structured-illumination microscope that is capable of 100-nm resolution at frame rates up to 11 Hz for several hundred time points. We demonstrate the microscope by video imaging of tubulin and kinesin dynamics in living Drosophila melanogaster S2 cells in the total internal reflection mode.

Original language	English
Pages (from-to)	339-342
Number of pages	4
Journal	Nature Methods
Volume	6
Issue number	5
DOIs	https://doi.org/10.1038/NMETH.1324
Publication status	Published - May 2009

Keywords

REFLECTION FLUORESCENCE MICROSCOPY
LOCALIZATION MICROSCOPY
SPINDLE
RESOLUTION
DYNAMICS
PROTEIN
KINETOCHORE
MITOSIS
LIMIT
FLUX

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10.1038/NMETH.1324

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title = "Super-resolution video microscopy of live cells by structured illumination",

abstract = "Structured-illumination microscopy can double the resolution of the widefield fluorescence microscope but has previously been too slow for dynamic live imaging. Here we demonstrate a high-speed structured-illumination microscope that is capable of 100-nm resolution at frame rates up to 11 Hz for several hundred time points. We demonstrate the microscope by video imaging of tubulin and kinesin dynamics in living Drosophila melanogaster S2 cells in the total internal reflection mode.",

keywords = "REFLECTION FLUORESCENCE MICROSCOPY, LOCALIZATION MICROSCOPY, SPINDLE, RESOLUTION, DYNAMICS, PROTEIN, KINETOCHORE, MITOSIS, LIMIT, FLUX",

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AU - Kner, Peter

AU - Chhun, Bryant B.

AU - Griffis, Eric R.

AU - Winoto, Lukman

AU - Gustafsson, Mats G. L.

PY - 2009/5

Y1 - 2009/5

N2 - Structured-illumination microscopy can double the resolution of the widefield fluorescence microscope but has previously been too slow for dynamic live imaging. Here we demonstrate a high-speed structured-illumination microscope that is capable of 100-nm resolution at frame rates up to 11 Hz for several hundred time points. We demonstrate the microscope by video imaging of tubulin and kinesin dynamics in living Drosophila melanogaster S2 cells in the total internal reflection mode.

AB - Structured-illumination microscopy can double the resolution of the widefield fluorescence microscope but has previously been too slow for dynamic live imaging. Here we demonstrate a high-speed structured-illumination microscope that is capable of 100-nm resolution at frame rates up to 11 Hz for several hundred time points. We demonstrate the microscope by video imaging of tubulin and kinesin dynamics in living Drosophila melanogaster S2 cells in the total internal reflection mode.

KW - REFLECTION FLUORESCENCE MICROSCOPY

KW - LOCALIZATION MICROSCOPY

KW - SPINDLE

KW - RESOLUTION

KW - DYNAMICS

KW - PROTEIN

KW - KINETOCHORE

KW - MITOSIS

KW - LIMIT

KW - FLUX

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M3 - Article

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VL - 6

SP - 339

EP - 342

JO - Nature Methods

JF - Nature Methods

IS - 5

ER -

Super-resolution video microscopy of live cells by structured illumination

Abstract

Keywords

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