Surface plasmon-enhanced aptamer-based fluorescence detection of cocaine using hybrid nanostructure of cadmium-free ZnSe/In2S3 core/shell quantum dots and gold nanoparticles

Kathryn Burton, Niamh Nic Daeid, Oluwasesan Adegoke (Lead / Corresponding author)

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)
76 Downloads (Pure)

Abstract

Cocaine abuse remains a global public health problem and a contributing factor to various social problems. In this work, we report on the development of a novel amphiphilic polymer (A-polym)-functionalized cadmium-free ZnSe/In2S3 core/shell quantum dots (QDs)-cationic cetyltrimethylammonium bromide (CTAB)-capped gold nanoparticle (AuNP) fluorescence nanoprobe for cocaine. ZnSe/In2S3 QDs were synthesized using the classic organometallic synthetic route for group II – VI semiconductor QDs and was surface-capped with organophosphorous-based ligands and organic-based stabilizers. A-polym, synthesized using an anhydride-capped polymer backbone and a nucleophilic agent, was used as a capping agent to coat and stabilize the QDs surface. The A-polym-coated QDs was electrostatically linked to cationic cetyltrimethylammonium bromide (CTAB)-capped gold nanoparticles (AuNPs) to form an A-polym-QDs-AuNP nanohybrid. The electrostatic interaction between the A-polym-coated QDs and CTAB-AuNPs quenched the fluorescence emission of the bound QDs. An MNS 4.1 anticocaine thiolated DNA aptamer (Aptm) was adsorbed on the A-polym-QDs-AuNP surface to form an Aptm-A-polym-QDs-AuNP nanoprobe. Upon interaction of cocaine with the Aptm-A-polym-QDs-AuNP nanoprobe, the fluorescence of the bound QDs was remarkably enhanced based on localized surface plasmon resonance-induced fluorescence emission signal. In general, the QDs functioned as the fluorophore reporter, CTAB-AuNP as a plasmon amplifier and the Aptm as the receptor. Under optimum reaction conditions, cocaine was selectively and quantitatively detected with a detection limit of 0.8 nM. Selectivity was achieved against other drugs and cocaine metabolites. The efficacy of the Aptm-A-polym-QDs-AuNP nanoprobe to detect cocaine in adulterated cocaine samples was successfully achieved.
Original languageEnglish
Article number114131
Number of pages13
JournalJournal of Photochemistry and Photobiology A: Chemistry
Volume433
Early online date8 Jul 2022
DOIs
Publication statusPublished - 1 Dec 2022

ASJC Scopus subject areas

  • General Physics and Astronomy
  • General Chemical Engineering
  • General Chemistry

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