TY - JOUR
T1 - Surfaces for hearts
T2 - Establishing the optimum plasma surface engineering methodology on polystyrene for cardiac cell engineering
AU - Kitsara, Maria
AU - Kontziampasis, Dimitrios
AU - Bolomiti, Efi
AU - Simon, Alexandre
AU - Dimitrakis, Panagiotis
AU - Miche, Antoine
AU - Kokkoris, Georgios
AU - Humblot, Vincent
AU - Agbulut, Onnik
N1 - © 2023 The Authors.
This work was supported by the LabEx REVIVE (ANR-10-LABX-73), the Ile-de-France Region in the framework of the DIM Respore, the Île-de-France network of Excellence in Porous Solids and the AFM-Téléthon (contract number: 22142). MK acknowledges personal funding from the LabEx REVIVE and DIM Respore too. The authors also acknowledge IMPC from Sorbonne University (Institut des Matériaux de Paris Centre, FR CNRS 2482) and the C’Nano projects of the Region Ilede-France, for Omicron XPS apparatus funding. The authors thank the personnel of the Photon Microscopy Facility of IBPS for helpful advice and technical assistance during microscopy image acquisition and analysis.
PY - 2023/5/30
Y1 - 2023/5/30
N2 - Plasma surface modification is a popular method for improving cell culture on surfaces, and polystyrene (PS) is literature's material of choice. This study identifies the optimum plasma treatment for promoting normal cardiac cell behaviour during culture. PS slides were plasma-treated with O
2, N
2, O
2 + N
2 and Ar + N
2 for 20 and 30 min in a reactive ion etcher (RIE). SEM reveals that O
2 and O
2 + N
2 plasmas create dual scale roughness, N
2 plasma creates oval-shaped structures, while Ar + N
2 exhibits no topography. Evaluation by XPS reveals an increase in the atomic percentage of oxygen for all treatments. Contact angle measurements agree as all treatments lead to hydrophilisation, with N
2 samples exhibiting long-term stability. Two sources of cells were used to identify the optimum plasma treatment for cardiac cell culture on PS. H9c2 cells exhibit optimal behaviour with N
2 and N
2 + Ar regarding viability, morphology, and focal adhesion contact. The same was observed for primary cardiomyocytes on N
2 samples. For purified cardiomyocytes, immunofluorescence revealed well-organised sarcomeric structure on N
2 samples, exhibiting clear improvement compared to control. SEM validated these findings, as cardiomyocytes on N
2-treated PS exhibited physiological, elongated shape. These findings provide solid evidence that the optimum treatment for PS is the use of N
2 plasma.
AB - Plasma surface modification is a popular method for improving cell culture on surfaces, and polystyrene (PS) is literature's material of choice. This study identifies the optimum plasma treatment for promoting normal cardiac cell behaviour during culture. PS slides were plasma-treated with O
2, N
2, O
2 + N
2 and Ar + N
2 for 20 and 30 min in a reactive ion etcher (RIE). SEM reveals that O
2 and O
2 + N
2 plasmas create dual scale roughness, N
2 plasma creates oval-shaped structures, while Ar + N
2 exhibits no topography. Evaluation by XPS reveals an increase in the atomic percentage of oxygen for all treatments. Contact angle measurements agree as all treatments lead to hydrophilisation, with N
2 samples exhibiting long-term stability. Two sources of cells were used to identify the optimum plasma treatment for cardiac cell culture on PS. H9c2 cells exhibit optimal behaviour with N
2 and N
2 + Ar regarding viability, morphology, and focal adhesion contact. The same was observed for primary cardiomyocytes on N
2 samples. For purified cardiomyocytes, immunofluorescence revealed well-organised sarcomeric structure on N
2 samples, exhibiting clear improvement compared to control. SEM validated these findings, as cardiomyocytes on N
2-treated PS exhibited physiological, elongated shape. These findings provide solid evidence that the optimum treatment for PS is the use of N
2 plasma.
KW - Plasma nanoscience
KW - Polystyrene
KW - Surface modification
KW - Nanobiotechnology
KW - Biointerfaces
KW - Cardiac cell engineering
UR - http://www.scopus.com/inward/record.url?scp=85149181914&partnerID=8YFLogxK
U2 - 10.1016/j.apsusc.2023.156822
DO - 10.1016/j.apsusc.2023.156822
M3 - Article
SN - 0169-4332
VL - 620
JO - Applied Surface Science
JF - Applied Surface Science
M1 - 156822
ER -