Sustained and dynamic inositol lipid metabolism inside and outside the immunological synapse

Patrick S. Costello; Maighread Gallagher; Doreen A. Cantrell

doi:10.1038/ni848

Sustained and dynamic inositol lipid metabolism inside and outside the immunological synapse

Patrick S. Costello
, Maighread Gallagher
, Doreen A. Cantrell

Research output: Contribution to journal › Article › peer-review

191 Citations (Scopus)

Abstract

T cell activation is triggered by several hours of contact with peptide-major histocompatibility (MHC) complexes on the surface of antigen-presenting cells (APCs). The nature and location of the sustained signal transduction pathways required for T cell activation are unknown. We show here that the production of phosphatidylinositol(3,4,5)triphosphate (PIP3) was dynamically sustained for hours as T cells responded to antigen. In addition, sustained elevation of PIP3 was essential for T cell proliferation. There was PIP3 accumulation in the T cell-APC contact zone and at the antipodal pole of the cell. The immune synapse is thus not the sole site of sustained signal transduction in activated T cells.

Original language	English
Pages (from-to)	1082-1089
Number of pages	8
Journal	Nature Immunology
Volume	3
Issue number	11
DOIs	https://doi.org/10.1038/ni848
Publication status	Published - Nov 2002

Keywords

1-Phosphatidylinositol 3-Kinase physiology
Antigen presentation physiology
Lymphocyte activation physiology
Membrane lipids metabolism
Phosphatidylinositol phosphates metabolism
Protein-serine-threonine kinases
Signal transduction physiology
T lymphocyte subsets immunology

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title = "Sustained and dynamic inositol lipid metabolism inside and outside the immunological synapse",

abstract = "T cell activation is triggered by several hours of contact with peptide-major histocompatibility (MHC) complexes on the surface of antigen-presenting cells (APCs). The nature and location of the sustained signal transduction pathways required for T cell activation are unknown. We show here that the production of phosphatidylinositol(3,4,5)triphosphate (PIP3) was dynamically sustained for hours as T cells responded to antigen. In addition, sustained elevation of PIP3 was essential for T cell proliferation. There was PIP3 accumulation in the T cell-APC contact zone and at the antipodal pole of the cell. The immune synapse is thus not the sole site of sustained signal transduction in activated T cells.",

keywords = "1-Phosphatidylinositol 3-Kinase physiology, Antigen presentation physiology, Lymphocyte activation physiology, Membrane lipids metabolism, Phosphatidylinositol phosphates metabolism, Protein-serine-threonine kinases, Signal transduction physiology, T lymphocyte subsets immunology",

author = "Costello, \{Patrick S.\} and Maighread Gallagher and Cantrell, \{Doreen A.\}",

note = "dc.publisher: Nature Publishing Group dc.description.sponsorship: Fondation pour la Recherche Medicale Cancer Research UK ",

year = "2002",

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doi = "10.1038/ni848",

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T1 - Sustained and dynamic inositol lipid metabolism inside and outside the immunological synapse

AU - Costello, Patrick S.

AU - Gallagher, Maighread

AU - Cantrell, Doreen A.

N1 - dc.publisher: Nature Publishing Group dc.description.sponsorship: Fondation pour la Recherche Medicale Cancer Research UK

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N2 - T cell activation is triggered by several hours of contact with peptide-major histocompatibility (MHC) complexes on the surface of antigen-presenting cells (APCs). The nature and location of the sustained signal transduction pathways required for T cell activation are unknown. We show here that the production of phosphatidylinositol(3,4,5)triphosphate (PIP3) was dynamically sustained for hours as T cells responded to antigen. In addition, sustained elevation of PIP3 was essential for T cell proliferation. There was PIP3 accumulation in the T cell-APC contact zone and at the antipodal pole of the cell. The immune synapse is thus not the sole site of sustained signal transduction in activated T cells.

AB - T cell activation is triggered by several hours of contact with peptide-major histocompatibility (MHC) complexes on the surface of antigen-presenting cells (APCs). The nature and location of the sustained signal transduction pathways required for T cell activation are unknown. We show here that the production of phosphatidylinositol(3,4,5)triphosphate (PIP3) was dynamically sustained for hours as T cells responded to antigen. In addition, sustained elevation of PIP3 was essential for T cell proliferation. There was PIP3 accumulation in the T cell-APC contact zone and at the antipodal pole of the cell. The immune synapse is thus not the sole site of sustained signal transduction in activated T cells.

KW - 1-Phosphatidylinositol 3-Kinase physiology

KW - Antigen presentation physiology

KW - Lymphocyte activation physiology

KW - Membrane lipids metabolism

KW - Phosphatidylinositol phosphates metabolism

KW - Protein-serine-threonine kinases

KW - Signal transduction physiology

KW - T lymphocyte subsets immunology

U2 - 10.1038/ni848

DO - 10.1038/ni848

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SN - 1529-2908

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SP - 1082

EP - 1089

JO - Nature Immunology

JF - Nature Immunology

IS - 11

ER -

Sustained and dynamic inositol lipid metabolism inside and outside the immunological synapse

Abstract

Keywords

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